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I-RT-LAMP Fluorenscent Master Mix HCB5205A Isithombe Esifakiwe
  • I-RT-LAMP Fluorenscent Master Mix HCB5205A

I-RT-LAMP Fluorenscent Master Mix


Inombolo yekati: HCB5205A

Iphakheji: 96RXN/960RXN/9600RXN

Lo mkhiqizo uqukethe isivimbeli sokusabela, i-RT-Enzymes Mix (i-Bst DNA polymerase kanye ne-reverse transcriptase engashisi), ama-Lyophilized Protectants kanye nezingxenye zikadayi ze-chromogenic.

Incazelo Yomkhiqizo

Imininingwane yomkhiqizo

Lo mkhiqizo uqukethe isigcinalwazi sokusabela, i-RT-Enzymes Mix (i-Bst DNA polymerase kanye ne-reverse transcriptase engashisi ukushisa), ama-Lyophilized Protectants kanyei-fluorescentizingxenye zodayi.Ibhafa yokusabela iqukethe i-Mg2+, i-dNTP nezinye izingxenye ezidingekayo zokukhulisa.Ukuze usebenzise, ​​vele uhlanganise i-Buffer, i-enzyme yokusabela, udayi we-fluorescent, i-primer bese wengeza isifanekiso, ukwengeza isivikeli se-lyophilized singasebenzisa ngokuqondile.Yayixhunywe ku-lyophilizer kanye ne-lyophilized, futhi kuphela ama-primers kanye nezifanekiso zengezwe lapho zisetshenziswa.Lesi sici sinokwethenjelwa okuphezulu kwe-amplification kanye nokuzwela.


  • Okwedlule:
  • Olandelayo:

  • Isakhi

    Isakhi

    I-HCB5205A-01

    HCB5205A-02

    HCB5205A-03

    Ibhafa yokukhulisa i-Loop-mediated

    1.2 mL

    4 mlx3

    12 mLx10

    I-RT-Enzymes Mix

    245 μL

    2.45 mL

    2.45 mL×10

    Isivikelo se-Lyophilized

    0.96 mL×2

    9.60 mL×2

    9.60 mL×20

    25× Udayi

    192 μL

    0.96 mL×2

    0.96 mL×20

     

    Izinhlelo zokusebenza

    Nge-DNA noma i-RNA isothermal amplification.

     

    Izimo Zokugcina

    Kuthuthwa ngeqhwa elomile, eligcinwe ku -25~ -15 ℃.Gwema ukuncibilika kweqhwa njalo, umkhiqizo usebenza izinyanga eziyi-12.

     

    Iphrothokholi

    1.Ncibilikisa isigcinalwazi esizosetshenziswa ekamelweni lokushisa.I-Vortex kafushane noma guqula amashubhu izikhathi ezimbalwa ukuze ixube kahle, bese i-centrifuge ukuqoqa uketshezi phansi kweshubhu.

    2.Ukulungiswa kwesistimu yokusabela.Lesi sici singalungiswa ngezinhlelo ezimbili zokusabela, ingxube ye-liquid reaction kanye ne-lyophilized system mix.

    1) Lungiselela ukuxubana kwe-liquid reaction

    Isakhi

    Ivolumu

    Ibhafa yokukhulisa i-Loop-mediated

    12.5 μL

    25× Udayi

    2 ml

    I-RT-Enzymes Mix

    2.55 μL

    10 × I-Primer Mixa

    5 μl

    Izifanekiso ze-DNA/RNA b

    × μL

    Amanzi Angenayo I-nuclease

    Kufika ku-50 μL

     

    2) I-Lyophilization system mix

    ① Lungiselela imiksi ye-lyophilized

    Isakhi

    Ivolumu

    Ibhafa yokukhulisa i-Loop-mediated

    12.5 μL

    Isivikelo se-Lyophilized

    20 μL

    25× Udayi

    2 ml

    I-RT-Enzymes Mix

    2.55 μL

    Amanzi Angenayo I-nuclease

    Kufika ku-50 μL

    ② I-Lyophilization: I-Mix elungisiwe i-lyophilized ohlelweni lwe-50μL

    ③ Lungiselela imiksi yokusabela

    Isakhi

    Ivolumu

    Ingxube ye-Lyophilized

    1 isiqephu

    10 × I-Primer Mixa

    5 μl

    Izifanekiso ze-DNA/RNA b

    × μL

    Amanzi Angenayo I-nuclease

    Kufika ku-50 μL

    Amanothi:

    1) a.10×I-Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Iluphu F/B;

    2) b.I-DEPC (i-soluble yamanzi) inconywa nge-nucleic acid templ.

    3)Ukusabela Kokukhulisa: Setha uhlelo olulandelayo ensimbini ye-PCR ekhanyayo (isb. i-ABI7500, njll.):

    Izinga lokushisa

    Isikhathi

    Imibuthano

    65 ℃

    60 isekhondi *qoqa i-FAM fluorescence

    30

     

    Amanothi

    1.Usawoti ungase uvele ngaphansi kweshubhu lebhafa, i-vortex kafushane noma uguqule amashubhu izikhathi ezimbalwa ukuze uxubane kahle ekamelweni lokushisa.

    2.Izinga lokushisa lokusabela lingenziwa kahle phakathi kuka-62 ℃ no-68 ℃ ngokwesimo sezinto zokuqala.

    3.Ukuhlolwa kufanele kufane, okuhlanganisa ukulungiswa kwesistimu yokusabela, i-lyophilization, nokucubungula isampula kanye nenqubo yokwengeza isampula;

    4.Ukuze ugweme ukungcola, kunconywa ukuthi ulungise isistimu yokusabela ebhentshini elihlanzeke kakhulu, kwelinye Engeza izifanekiso ku-fume hood yegumbi ukuze ugweme ukugxambukela okungamanga.

     

     

    Bhala umyalezo wakho lapha futhi usithumelele wona