I-Taqman Multiplex qPCR Premix
Inombolo yekati: HCB5061B
I-Taqman Multiplex qPCR Premix master mix isizukulwane esisha se-antibody eguquliwe i-Taq DNA Polymerase fluroescence quantitative qPCR ingasetshenziswa ku-lyophilization reagent.Ifanele i-FAM/HEX/TET/ JOE/ROX njll. Ama-probe anamalebula kabili (futhi afanele ama-Molecular Beacon probes).I-reagent iqukethe i-hotstart Fast Taq DNA Polymerase, i-dNTP njll. Ingxenye engomiswa mahhala ngqo emshinini.Umkhiqizo awuqukethe odayi abalungisiwe be-ROX futhi ulungele amamodeli e-PCR ahlukahlukene.
Izingxenye
I-Lyo-Ready TaqMan qPCR Mix
Izimo Zokugcina
Lo mkhiqizo kufanele ugcinwe ku -25~-15 ℃ kude nokukhanya unyaka ongu-1.
Iziyalezo
1.Isistimu yokusabela
Izingxenye | Ivolumu (μL) | Ukugxila kokugcina |
I-Enzyme Mix | 20 | - |
I-Primer Primer (10μM) | x | 0.1-0.5μM |
I-Probe Primer (10μM) | x | 50-250nM |
I-DNA Tamplate | 1-25 | - |
dd H2O | kufika ku-25 | - |
2.Uhlelo lokusabela
Isinyathelo somjikelezo | Temp. | Isikhathi | Imijikelezo |
I-denaturation yokuqala | 95℃ | 5 imiz | 1 |
Ukusabela kokukhulisa | 95℃ | 15 isekhondi | 45 |
I-Annealing/Extension | 60℃ | 30 isekhondi |
Amanothi:Ezimweni ezikhethekile izinga lokushisa le-annealing/extension lingalungiswa ngokuya nge-primer Tm value, 60°C kuyanconywa.
Inqubo ye-Lyophilization
Inqubo | Temp. | Setha isikhathi | Isikhathi sobude | Ingcindezi |
Ukubanda kwangaphambili | -45 ℃ | 10 imiz | 60 imiz | - |
Ukomisa Okuyisisekelo | -45 ℃ | 10 imiz | 60 imiz | 0.01 umbhoshongo |
-40 ℃ | 10 imiz | 600min | 0.01 umbhoshongo | |
-35 ℃ | 10 imiz | 120 min | 0.01 umbhoshongo | |
-30 ℃ | 20 min | 60 imiz | 0.01 umbhoshongo | |
-20 ℃ | 20 min | 60 imiz | 0.01 umbhoshongo | |
-10 ℃ | 20 min | 60 imiz | 0.01 umbhoshongo | |
0℃ | 20 min | 60 imiz | 0.01 umbhoshongo | |
Ukomisa okwesibili | 5℃ | 10 imiz | 20 min | 0.01 umbhoshongo 0.01 umbhoshongo |
10℃ | 10 imiz | 20 min | ||
15℃ | 10 imiz | 20 min | 0.01 umbhoshongo | |
20℃ | 10 imiz | 20 min | 0.01 umbhoshongo | |
25℃ | 10 imiz | 240min | 0.01 umbhoshongo |
Amanothi
1. Lo mkhiqizo owokusetshenziswa ucwaningo kuphela.
2. Sicela usebenzise amajazi elebhu namagilavu alahlwayo, ukuze uphephe.