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I-Taqman Multiplex qPCR Premix HCB5061B Isithombe Esifakiwe
  • I-Taqman Multiplex qPCR Premix HCB5061B

I-Taqman Multiplex qPCR Premix


Inombolo yekati: HCB5061B

Iphakheji: 100RXN/1000RXN/10000RXN

I-Taqman Multiplex qPCR Premix master mix isizukulwane esisha se-antibody eguquliwe i-Taq DNA Polymerase fluroescence quantitative qPCR ingasetshenziswa ku-lyophilization reagent.

Incazelo Yomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati: HCB5061B

I-Taqman Multiplex qPCR Premix master mix isizukulwane esisha se-antibody eguquliwe i-Taq DNA Polymerase fluroescence quantitative qPCR ingasetshenziswa ku-lyophilization reagent.Ifanele i-FAM/HEX/TET/ JOE/ROX njll. Ama-probe anamalebula kabili (futhi afanele ama-Molecular Beacon probes).I-reagent iqukethe i-hotstart Fast Taq DNA Polymerase, i-dNTP njll. Ingxenye engomiswa mahhala ngqo emshinini.Umkhiqizo awuqukethe odayi abalungisiwe be-ROX futhi ulungele amamodeli e-PCR ahlukahlukene.


  • Okwedlule:
  • Olandelayo:

  • Izingxenye

    I-Lyo-Ready TaqMan qPCR Mix

     

    Izimo Zokugcina

    Lo mkhiqizo kufanele ugcinwe ku -25~-15 ℃ kude nokukhanya unyaka ongu-1.

     

    Iziyalezo

    1.Isistimu yokusabela

    Izingxenye

    Ivolumu (μL)

    Ukugxila kokugcina

    I-Enzyme Mix

    20

    -

    I-Primer Primer (10μM)

    x

    0.1-0.5μM

    I-Probe Primer (10μM)

    x

    50-250nM

    I-DNA Tamplate

    1-25

    -

    dd H2O

    kufika ku-25

    -

     

    2.Uhlelo lokusabela

    Isinyathelo somjikelezo

    Temp.

    Isikhathi

    Imijikelezo

    I-denaturation yokuqala

    95℃

    5 imiz

    1

    Ukusabela kokukhulisa

    95℃

    15 isekhondi

    45

    I-Annealing/Extension

    60℃

    30 isekhondi

     Amanothi:Ezimweni ezikhethekile izinga lokushisa le-annealing/extension lingalungiswa ngokuya nge-primer Tm value, 60°C kuyanconywa.

     

    Inqubo ye-Lyophilization

    Inqubo

    Temp.

    Setha isikhathi

    Isikhathi sobude  

    Ingcindezi

    Ukubanda kwangaphambili

    -45 ℃

    10 imiz

    60 imiz

    -

     

    Ukomisa Okuyisisekelo

    -45 ℃

    10 imiz

    60 imiz

    0.01 umbhoshongo

    -40 ℃

    10 imiz

    600min

    0.01 umbhoshongo

    -35 ℃

    10 imiz

    120 min

    0.01 umbhoshongo

    -30 ℃

    20 min

    60 imiz

    0.01 umbhoshongo

    -20 ℃

    20 min

    60 imiz

    0.01 umbhoshongo

    -10 ℃

    20 min

    60 imiz

    0.01 umbhoshongo

    0℃

    20 min

    60 imiz

    0.01 umbhoshongo

     

    Ukomisa okwesibili

    5℃

    10 imiz

    20 min

    0.01 umbhoshongo

    0.01 umbhoshongo

    10℃

    10 imiz

    20 min

    15℃

    10 imiz

    20 min

    0.01 umbhoshongo

    20℃

    10 imiz

    20 min

    0.01 umbhoshongo

    25℃

    10 imiz

    240min

    0.01 umbhoshongo

     

     Amanothi

    1. Lo mkhiqizo owokusetshenziswa ucwaningo kuphela.

    2. Sicela usebenzise amajazi elebhu namagilavu ​​alahlwayo, ukuze uphephe.

     
    Bhala umyalezo wakho lapha futhi usithumelele wona