Ikhithi Yokuhlola I-Glycohemoglobin A1c (HbA1c).
Izinzuzo
● Ukunemba okuphezulu
● Ikhono eliqinile lokulwa nokuphazamiseka
● Ukuzinza okuhle
Isakhiwo Samakhemikhali
Izinhlelo zokusebenza
Ukuhlolwa kwe-in vitro kokunqunywa kwenani lokugxila kwe-HbA1c egazini lomuntu lonke kumasistimu e-photometric.I-HbA1c iwumkhiqizo we-hemoglobin (Hb) okhiqiza ukusabela kwe-glycation kancane kancane nokuqhubekayo okungeyona i-enzymatic ngaphansi kweglucose ephezulu yegazi.IGlucose iguqula i-hemoglobin ikakhulukazi ensaleni yayo ye-n-terminal valine ukuze yakhe i-glycated hemoglobin.Ngaphansi kwezimo ezijwayelekile zomzimba, ukukhiqizwa kwemikhiqizo ye-non-enzymatic glycosylation reaction ilingana kahle nokuhlangana kwama-reactants.Njengoba ukugcwala kwe-hemoglobin kuhlala kuzinzile, amazinga e-glycosylation ancike kakhulu ekugxilweni kweglucose futhi ahlobene nobude be-hemoglobin nokuchayeka kweglucose.Ngakho-ke, i-HbA1c iyinkomba enhle yezinga likashukela wegazi elimaphakathi wezinyanga ezi-2–3 ezedlule.
Isimiso
Ngaphansi kwesenzo se-protease, i-n-terminal ye-β chain ku-HbA1c iyanqanyulwa bese kukhishwa ama-dipeptide e-glycosylated.Ekuphenduleni kokuqala, ukugxila kwe-Hb kungatholwa ngokulinganisa ukumuncwa okungu-480 nm.Ekuphenduleni kwesibili, i-fructosyl peptide oxidase (FPOX) isebenza kuma-dipeptides e-glycosylated ukuze ikhiqize i-hydrogen peroxide engasabela nama-ejenti e-chromogenic ukuze ikhiqize ukumunca ku-660nm phambi kwe-peroxidase, khona-ke ukugxila kwe-HbA1c kungatholakala ngokulinganisa ukumuncwa kwe-HbA1c. 660nm.Ngokuya ngokugxilisa kwe-HbA1c okutholiwe nokugxiliswa kwe-Hb, iphesenti le-HbA1c(HbA1c%) lingabalwa.
Kuyasebenza
I-Hitachi 7180/7170/7060/7600 i-automatic biochemical analyser, Abbot 16000, OLYMPUS AU640automatic biochemical analyzer
Ama-reagents
| Izingxenye | Izigxivizo |
| I-Reagent 1(R1) | |
| Ibhafa ye-Good | 100 mmol/L |
| I-PRK | 500KU/L |
| DA-67 | 10 mmol/L |
| Ama-reagents 2 (R2) | |
| Ibhafa ye-Good | 100 mmol/L |
| I-Fructosyl peptide oxidase | 50 KU/L |
| I-Reagent 3(R3) | |
| Ibhafa ye-Good | 100 mmol/L |
Ezokuthutha nokugcina
Ezokuthutha:I-ambient
Isitoreji Nokuzinza:
Kuze kube yidethi yokuphelelwa yisikhathi eboniswe kulebula, uma igcinwe ingavuliwe ku-2-8℃ futhi ivikelwe ekukhanyeni.Uma sezivuliwe, ama-reagents azinzile izinsuku ezingu-28 lapho efakwe efrijini ku-analyzer noma esiqandisini.
Ukungcoliswa kwama-reagents kufanele kugwenywe.Ungawashiyi ama-reagents.
Uma isincibilikile, i-calibrator izinzile izinsuku eziyi-15 ku-2-8 ℃, isilawuli sizinzile izinsuku eziyi-7 ku-2-8 ℃, musa iqhwa.
Impilo Yeshelufu:1 unyaka
