I-Fructosyl-peptide Oxidase (FPOX)
Incazelo
I-enzyme iyasiza ekunqumeni i-fructosyl-peptide ne-fructosyl-L-amino acid.
Isakhiwo Samakhemikhali
Isimiso Sokusabela
I-Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe e-amorphous, i-lyophilized |
| Umsebenzi | ≥4U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| IsiCatalase | ≤0.01% |
| I-ATPase | ≤0.005% |
| I-glucose oxidase | ≤0.03% |
| I-Cholesterol oxidase | ≤0.003% |
Ezokuthutha nokugcina
Ezokuthutha: I-ambient
Isitoreji:Gcina ku -20°C(Isikhathi eside), 2-8°C (isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo:2 iminyaka
Umlando wokuthuthuka
Enye yezinkomba ezisetshenziswa ekuxilongweni kwesifo sikashukela i-glycated hemoglobin (HbA1c).Ukukalwa kwe-HbA1c kusetshenziswa ama-enzyme kulungele ukucubungula izinombolo eziningi zezibonelo, futhi kuyabiza.Ngakho-ke, sekunesikhathi eside kukhona ucingo oluqinile oluvela kubasebenzi bezempilo mayelana nokuthuthukiswa kokuhlolwa kwe-enzyme enjalo.Ngakho-ke, sithuthukise ukuhlolwa okusha sisebenzisa "indlela ye-dipeptide".Ngokukhethekile, sithole “i-Fructosyl-peptide Oxidase” (FPOX) engasetshenziswa njenge-enzyme yalokhu kuhlolwa.Lokhu kwenze kwaba lula impumelelo yethu ekuzuzeni owokuqala emhlabeni ngokwenza kube ngokoqobo ukuhlolwa kwe-enzyme ye-HbA1c.Le "ndlela ye-dipeptide" isebenzisa i-Protease (i-Proteolytic enzyme) ukuze iphule i-HbA1c egazini, bese ikala amazinga e-dipeptide e-saccharified akhiqizwa kusetshenziswa i-FPOX.Le ndlela ihlangabezane nokwamukelwa okuhle kakhulu ngenxa yokufaneleka kwayo ukuba ilula, ingabizi futhi isheshe, futhi i-HbA1c yokukala i-reagent esebenzisa i-FPOX manje isisetshenziswa emhlabeni wonke.
