I-Homocysteine(HCY)
Incazelo
I-Homocysteine(HCY) isetshenziselwa ukuthola i-homocysteine egazini lomuntu.I-Homocysteine (Hcy) iyi-amino acid enesulfure ekhiqizwa yi-methionine metabolism.I-80% ye-Hcy iboshelwe kumaprotheni ngokusebenzisa izibopho ze-disulfide egazini, futhi ingxenye encane kuphela ye-homocysteine yamahhala ebamba iqhaza ekujikelezeni.Amazinga e-Hcy ahlobene eduze nezifo zenhliziyo.iyisici esibalulekile esiyingozi yesifo senhliziyo nemithambo yegazi.Ukwanda kwe-Hcy egazini kuvuselela udonga lwemithambo yegazi ukuze lubangele umonakalo emithanjeni yegazi, okuholela ekuvuvukeni nasekubunjweni kwama-plaque odongeni lomkhumbi, okugcina kuholela ekuvinjweni kokugeleza kwegazi enhliziyweni.Ezigulini ezine-hyperhomocystinuria, ukukhubazeka okukhulu kwezakhi zofuzo kuthinta i-Hcy metabolism, okuholela ku-hyperhomocysteinemia.Ukukhubazeka okuncane kwezakhi zofuzo noma ukuntuleka kokudla okunomsoco kwamavithamini B kuzohambisana nokuphakama okulinganiselwe noma okuncane kwe-Hcy, okuzokwandisa ingozi yesifo senhliziyo.I-Hcy ephakeme ingabangela nokukhubazeka kokuzalwa okufana nokukhubazeka kwe-neural tube kanye nokukhubazeka kokuzalwa.
Isakhiwo Samakhemikhali
Umgomo wokuhlola
I-Hcy ene-oxidized iguqulelwa ku-Hcy yamahhala, futhi i-Hcy yamahhala isabela nge-serine ngaphansi kwe-catalysis ye-CBS ukuze ikhiqize i-L-cystathionine.I-L-cystathionine ikhiqiza i-Hcy, i-pyruvate ne-NH3 ngaphansi kwe-catalysis ye-CBL.I-pyruvate ekhiqizwa yilokhu kusabela komjikelezo ingatholwa nge-lactate dehydrogenase LDH ne-NADH, futhi izinga lokuguqulwa le-NADH liye ku-NAD lilingana ngokuqondile nokuqukethwe kwe-Hcy kusampula.
Ezokuthutha nokugcina
Ezokuthutha:2-8°C
Isikhathi sokugcina nesokuqinisekisa:Ama-reagents angavuliwe kufanele agcinwe ku-2-8 ° C ebumnyameni, futhi isikhathi sokufaneleka yizinyanga ezingu-12;ngemuva kokuvula, ama-reagents kufanele agcinwe ebumnyameni ku-2-8 ° C, futhi isikhathi sokuqinisekisa siyinyanga engu-1 ngaphansi kwesimo sokungangcoli;ama-reagents akufanele aqandiswe.
Qaphela
Izidingo zesampula: Isampula yi-serum entsha noma i-plasma (i-heparin anticoagulation, i-0.1mg ye-heparin inganqanda ukuqina kwegazi elingu-1.0ml).Sicela ufake i-plasma i-centrifuge ngokushesha ngemva kokuqoqwa kwegazi, noma uyifake esiqandisini futhi uyibeke phakathi kwehora elingu-1.
