I-T4 RNA ligase
Incazelo
Lo mkhiqizo uyi-T4 RNA ligase encike ku-ATP (T4 RNA Ligase I) evezwe kabusha yi-E. coli.Le enzyme igqugquzela ukwakheka kwezibopho ze-phosphodiester phakathi kwe-oligonucleotides, i-RNA ene-stranded single kanye ne-DNA intermolecular/ intra-molecular 5'-PO4 kanye ne-3'-OH.
Incazelo Yeyunithi
Iyunithi eyodwa ichazwa njengenani le-enzyme edingekayo ukuze kuguqulwe i-nanomole engu-1 ye-5´-[³²P] rA16 ibe ifomu elimelana ne-phosphatase emizuzwini engu-30 ku-37°C.
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Umsebenzi we-Enzyme | 10 U/μL |
| Umsebenzi Wamaprotheni | ≥ 95% |
| I-Nickase | Ayibonakali |
| Umsebenzi we-RNase | Ayibonakali |
| Ecoli gDNA | ≤1ikhophi/50U |
| I-Endotoxin | I-LCL-Test,≤10EU/mg |
Ezokuthutha nokugcina
Ezokuthutha:Iqhwa elomile
Isitoreji:Gcina ku-25~-15°C (gwema ukuqhwa okuphindaphindiwe nokuncibilika)
Impilo yokuhlola kabusha enconyiwe:2 iminyaka
imikhiqizo ehlobene
Bhala umyalezo wakho lapha futhi usithumelele wona
