I-BspQI Restriction Endonuclease
Incazelo
I-BspQI ingavezwa kabusha ngokuhlanganyela ku-E. coli ekwazi ukubona amasayithi athile futhi akhiqizwa ngaphansi kwamazinga e-GMP.Isetshenziswa kakhulu ekwenzeni imephu yofuzo, i-cloning neminye imikhakha.Le enzyme isika ngokushesha i-DNA ukuze i-gene linearization ephumelelayo.
5'······GGTC(N)1······3'
3'······CCAGAG(N)4······5'
Isakhiwo Samakhemikhali
Incazelo Yeyunithi
Iyunithi eyodwa ichazwa njengenani le-enzyme edingekayo ukuze kugaywe i-1 µg ye-λ DNA ehoreni elingu-1 ku-50°C kuvolumu yokusabela ephelele engu-50 µl.
Izici Zomkhiqizo
• I-endonuclease evimbelayo inomsebenzi ophezulu we-enzymatic futhi iqeda ukugaya ngokushesha.
• Umsebenzi ophansi we-endonuclease ongaqondile ukuqinisekisa ukusika okunembile "kwe-scalpel".
• Alukho uhlelo lwe-BSA, olunciphisa ukungcoliswa komthombo wokushisa.
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Amaprotheni Purity | ＞98% |
| Izinsalela ze-Protease | Akukho okutholiwe |
| Ukuxhumana nokutholwa kokusika | Ukusebenza kahle kokugaya ukudla ＞95% |
| DNase | Akukho okutholiwe |
| Nicaknase | Akukho okutholiwe |
Ezokuthutha nokugcina
Ezokuthutha:Kuthunyelwe ngaphansi kuka-0 °C
Isitoreji:Gcina ku -20 °C
Impilo yokuhlola kabusha enconyiwe:2unyaka
