Ikhithi yokuhlola ye-DNase (Fluorescence)
Incazelo
Ikhithi yokuthola i-DNase isuselwe ocwaningweni lwe-DNA olunelebuli ye-fluorophore.Uma isampula ingenawo umsebenzi we-DNase, i-probe izinzile futhi ayikhiqizi isignali ye-fluorescent;uma isampula iqukethe umsebenzi we-DNase, i-probe yonakaliswa, okuholela esignali ye-fluorescence ethuthukisiwe kancane kancane;izinga lokukhuphuka kwesignali ye-fluorescence lihlotshaniswa kahle nenombolo kanye nomsebenzi wama-enzyme.Sebenzisa isifundi se-fluorescence microplate ukuze ulinganise kubude begagasi be-ex/em=485/525nm ukuze unqume ukuthi isampula lingcolisiwe yini yi-DNase
Isakhiwo Samakhemikhali
Ukwakhiwa kwekhithi
Igama | 48Isivivinyo | 192Isivivinyo |
10×isixazululo sokusabela | 0.5mL | 2.0mL |
I-DNA probe | 1 ithubhu | 1 ithubhu |
Ibhafa ye-TE | 0.5mL | 2.0mL |
I-DNase I ejwayelekile (2U/μL) | 10μL | 20μL |
I-Standard Dilution Buffer | 6ml | 12ml |
Amanzi angenayo i-DNase | 25ml | 25ml |
DNase RNase kude | 50ml | 50ml |
Ukucaciswa
Izinto Zokuhlola | Imininingwane |
Ukubukeka | Ukupakisha okuphelele futhi akukho ukuvuza okuwuketshezi |
Umkhawulo wokutholwa | I-DNase I: 1.25x10-6U/μL |
Ukunemba | I-Intra assay CV ≤ 10% |
Ezokuthutha nokugcina
Ezokuthutha:Iqhwa elomile
Isitoreji:Gcina ku-25~-15°C (gwema ukuqhwa okuphindaphindiwe nokuncibilika)
Impilo yokuhlola kabusha enconyiwe:1 unyaka