I-Uracil DNA Glycoylase (I-Glycerol-free)
Incazelo
I-Thermosensitive UDG (uracil-DNA glycosylase) ingakwazi ukuvuselela i-hydrolysis yesisekelo se-uracil seketango le-DNA eliqukethe i-uracil kanye nebhondi ye-N-glycosidic yomgogodla we-sugar-phosphate ukuze kukhululwe i-uracil yamahhala.Uma kuqhathaniswa nama-enzyme ajwayelekile e-UDG, ama-enzyme e-UDG anciphisa ukushisa agwema umsebenzi ongase ube yinsalela wama-enzyme avamile e-UDG ngemva kokungasebenzi, angehlisa izinga lemikhiqizo yokukhulisa equkethe i-dU ekamelweni lokushisa.Lo mkhiqizo usebenza ekamelweni lokushisa futhi uyazwela futhi ujwayele ukungasebenzi.
Isakhiwo Samakhemikhali
Ukucaciswa
| I-enzyme | I-Glycosylase |
| Ibhafa Ehambisanayo | Ibhafa Yesitoreji |
| Ukushisa Ukungasebenzi | 50°C, imizuzu eyi-10 |
| Incazelo Yeyunithi | Iyunithi eyodwa (U) ichazwa njengenani le-enzyme edingekayo ukuze kusungulwe i-hydrolysis ye-1 μg dU equkethe i-dsDNA ngemizuzu engama-30 ku-25°C. |
Izinhlelo zokusebenza
Susa ukungcoliswa kwe-aerosol yomkhiqizo we-PCR equkethe i-dU.
Ukususwa kwezisekelo ze-uracil kusuka ku-DNA enomucu owodwa noma ephindwe kabili
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -15℃ ~ -25℃
Impilo ye-Shief:1 iminyaka
