I-Hs Taq DNA Polymerase (i-Glycerol-free)

Inombolo ye-CAS: 9012-90-2 EC inombolo.: 2.7.7.7

Iphakheji: 1000U 5000U 50000U

Thumela Uphenyo

Incazelo Yomkhiqizo

Izinzuzo

Umsebenzi we-Taq enzyme ungakhululwa ngokuphelele ngokushisisa ku-95°C amasekhondi angu-30

Ukuzwela okuphezulu kokukhulisa nokucacisa

Ihambisana nezinhlelo ezihlukahlukene ze-PCR/qPCR

Incazelo

Isiqalo esishisayo i-Taq DNA Polymerase(i-Glycerol-free) iyi-antibody eshisayo yokuqala i-Taq DNA polymerase eklanyelwe ukwenza ama-lyophilized assays.I-Taq DNA polymerase iyi-enzyme ye-Taq eqala ukushisa etholwe ngokuxuba i-Taq antibody ne-Taq DNA polymerase ngesilinganiso esithile.Ngokusekelwe ekuzinzeni okushisayo kwe-Taq antibody, i-Taq DNA polymerase isengagcina ukuvalwa okuqinile kokungu-55°C, ukuze ukukhulisa okungaqondile kucindezelwe kube sezingeni eliphansi ngesikhathi sokuxutshwa kwesampula nezigaba zokushisisa uhlelo.Uma ukusabela kugcinwa ku-95°C isikhathi esingaphezu kwamasekhondi angu-30, i-antibody ye-Taq ayisebenzi ngokuphelele, futhi umsebenzi we-enzyme ye-Taq ukhululwa ngokuphelele, okuqinisekisa ukuthi uhlelo lwe-PCR lunokuzwela okuphezulu kokukhulisa nokucaciswa.

Isakhiwo samakhemikhali

Ukucaciswa

Izinto zokuhlola	Imiphumela
Amaprotheni Purity	≥95%
Umphumela Wokuvimbela	≥99%
Umsebenzi we-Exonuclease	Ayitholakali
Umsebenzi we-Nickase	Ayitholakali
Umsebenzi we-Rnase	Ayitholakali
Ukuzwela kokukhulisa	Dlula

Izinhlelo zokusebenza

I-Hs Taq DNA Polymerase Glycerol Free yenzelwe izinhlelo zokusebenza ezifaka i-automation kanye nokomisa iqhwa.Ukwakhiwa kwayo kwamahhala kwe-Glycerol kuzifanela kahle izinhlelo zokusebenza ze-PCR ezizenzakalelayo, noma lapho ukufakwa kwamapayipi okunembile kwamavolumu amancane kubalulekile.

Okwenziwa I-Glycerol

I-Glycerol ivamise ukuba yingxenye enkulu yesivikelo sokugcina sama-enzyme, futhi isebenza njenge-cryoprotectant.I-Glycerol iphazamisa ukwakheka kwamanzi futhi yenza isigcinalwazi sifane neseli, ngaleyo ndlela kusimamise i-polymerase.I-Glycerol iwuketshezi olune-viscous kakhulu ngakho-ke inzima futhi idla isikhathi ukuze ifake ipayipi ngokunembile, ikakhulukazi emiqulwini emincane.Njengomphumela, ukufaka i-glycerol ngepayipi ezinqubweni ezizenzakalelayo ezisizwa ngamarobhothi cishe kuyinselelo engaxazululeki.Ngaphezu kwalokho, ukuba khona kwe-glycerol ku-enzyme buffer kwenza ukomiswa kweqhwa kungenzeki.