Isinyathelo esisodwa se-RT-qPCR probe kit
Incazelo
I-U+ One Step RT-qPCR Probe Kit (i-Glycerol-free) iyi-agent ye-RT-qPCR yesinyathelo esisodwa engena-glycerol esebenzisa i-RNA njengesifanekiso (njengegciwane le-RNA), elifanele ukuthuthukiswa nokuklanywa kwemikhiqizo e-lyophilized.Lo mkhiqizo uhlanganisa ukusebenza okuphezulu kwesinyathelo esisodwa esizinikezele se-Reverse Transcriptase kanye ne-Champagne Taq DNA Polymerase eqala ukushisa, enesikhuseli samakhemikhali esomisa kahle esilungiselelwe kahle, esisebenza kahle kangcono ekukhuliseni, ibhalansi kanye nokucaciswa okuthile, futhi ehambisana nokumiswa okungafani kweqhwa. izinqubo.Ngaphezu kwalokho, isistimu ye-dUTP/UDG yokulwa nokungcoliswa ifakwa ku-reagent, engasebenza ekamelweni lokushisa, isuse umthelela wokungcoliswa komkhiqizo wokukhulisa i-qPCR, futhi iqinisekise ukunemba kwemiphumela.
Izimiso Eziyisisekelo ze-RT-qPCR
Ukucaciswa
| Izinto Zokuhlola | Ukucaciswa | Umphumela |
| (SDS IKHASI) Ukuhlanzeka Kwesitoko Se-Enzyme(SDS IKHASI) | ≥95% | Dlula |
| Umsebenzi we-Endonuclease | Ayitholakali | Dlula |
| Umsebenzi we-Exodulease | Ayitholakali | Dlula |
| Umsebenzi we-Rnase | Ayitholakali | Dlula |
| I-Residual E.coli DNA | ＜1 amakhophi/60 | Dlula |
| I-Functional Assay-System | 90%≤110% | Dlula |
Izingxenye
| Izingxenye | 100rxns | 1,000rns | 5,000 rxns |
| I-ddH2O yamahhala ye-RNase | 2*1ml | 20ml | 100ml |
| 5*isinyathelo esisodwa sokuxuba | 600μl | 6*1ml | 30ml |
| Isinyathelo esisodwa sokuxuba i-enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX inkomba Udayi 1 | 60μl | 600μl | 3*1ml |
| 50* ROX inkomba Udayi 2 | 60μl | 600μl | 3*1ml |
a.I-One-step Buffer ihlanganisa i-dNTP Mix ne-Mg2+.
b.I-Enzyme Mix ikakhulukazi iqukethe okuphambene
transcriptase, Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kanye ne-RNase inhibitor.
c.Isetshenziselwa ukulungisa iphutha lezinhlayiya ze-fluorescene phakathi kwemithombo ehlukene.
c.I-ROX: Udinga ukukhetha ukulinganisa ngokuya ngemodeli yethuluzi lokuhlola.
Izinhlelo zokusebenza
Ukutholwa kwe-QPCR
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -20 ℃.
I-Shief Life:Izinyanga ezingu-18
