I-Multiplex One Step RT-qPCR Probe Kit (UDG Plus)
Incazelo
I-Multiplex One Step RT-qPCR Kit (UDG Plus) iyikhithi yokuthola ngokushesha esekelwe ku-RT-qPCR elungele i-PCR ye-single-plex noma ye-multi-plex quantitative esebenzisa i-RNA njengesifanekiso (njengegciwane le-RNA).Isetshenziselwa ukukhulisa i-DNA enezintambo ezimbili kusukela kuzifanekiso ze-RNA ezinomucu owodwa ukuze kuvunyelwe ukulinganisa kwesikhathi sangempela okuhlosiwe kwe-RNA.Esinyathelweni sokuhlehla sokuloba i-reverse transcriptase ihlanganisa ama-molecule e-DNA anestranded single (cDNA) ehambisanayo nesifanekiso se-RNA.
Inqubo yokusabela
Izingxenye
| Izingxenye | 200rxns | 1,000rns | 10,000 rxns |
| I-ddH2O yamahhala ye-RNase | 3*1ml | 15ml | 150ml |
| 5*isinyathelo esisodwa sokuxuba | 800μl | 4*1ml | 40ml |
| Isinyathelo esisodwa sokuxuba i-enzyme | 200μl | 1ml | 10ml |
| 50* ROX inkomba Udayi 1 | 80μl | 400μl | 4*1ml |
| 50* ROX inkomba Udayi 2 | 80μl | 400μl | 4*1ml |
a.I-One-step Buffer ihlanganisa i-dNTP Mix ne-Mg2+.
b.I-Enzyme Mix ngokuyinhloko iqukethe i-reverse transcriptase, i-Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kanye ne-RNase inhibitor.
c.Isetshenziselwa ukulungisa iphutha lezinhlayiya ze-fluorescene phakathi kwemithombo ehlukene.
c.I-ROX: Udinga ukukhetha ukulinganisa ngokuya ngemodeli yethuluzi lokuhlola.
Izinhlelo zokusebenza
Ukutholwa kwamagciwane
ukuxilonga kanye nocwaningo lwesimila
Ukutholwa kwezifo zezilwane
Ukuxilongwa kokuqala kwezifo zofuzo
Ukutholwa kwama-microorganisms e-pathogenic ekudleni
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -30 ~ -15℃.
Impilo ye-Shief:1 iminyaka
