Ingxube ye-dNTP(25mM ngayinye/10mM ngayinye)
Incazelo
Lo mkhiqizo uyisixazululo esiwuketshezi esingenambala, i-dNTP Mix iyisixazululo esixutshwe ngaphambili se-dATP, i-dCTP, i-dGTP ne-dTTP, ngayinye ene-concentration ye-100 mmol/L, ilungele ukuhlolwa okujwayelekile kwe-molecular biology njengokukhulisa i-PCR, i-PCR yesikhathi sangempela, i-cDNA. noma i-DNAsynthesis evamile, ukulandelana kwe-DNA nokulebula.Ingahlanjululwa ngamanzi ahlanzekile kakhulu, futhi ilungiswe ku-pH 7.0 ngesisombululo se-NaOH esihlanzekile, ngobumsulwa ≥ 99% (HPLC).Ngemva kokutholwa, ayiqukethe i-DNase, i-RNase ne-phosphotase.Ingasetshenziswa ngokuqondile ekuphenduleni okujwayelekile kwamangqamuzana ezinto eziphilayo njenge-PCR.
Ukucaciswa
| Izinto zokuhlola | Imininingwane |
| Ukubukeka | Sula isixazululo esingenambala |
| Ukugxila | 25mM ngayinye/10mM ngayinye |
| Ubumsulwa (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.5 |
| Ukulawulwa KwekhwalithiLokhu kulungiselelwa akukho ekungcoleni kwe-DNase Le dNTP eningi ihlolwe ukusebenza nge-Taq DNA polymerase kanye ne-Pfu DNA polymerase. Le ngxenye ye-d NTP ihlolwe ukusebenza nge-Q-PCR | |
Izinhlelo zokusebenza
I-PCR yebanga elide (20 KB)
I-cDNA synthesis kanye ne-RT-PCR
I-PCR yesikhathi sangempela
I-PCR ejwayelekile
Inani eliphakeme kakhulu lama-PCR
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -25～-15℃
Idethi yokuhlola kabusha enconyiwe:iminyaka engu-2
