I-Ribonuclease A (RNase A)

I-Ribonuclease A (RNase A) iyi-polypeptide enomucu owodwa equkethe amabhondi angu-4 e-disulfide enesisindo samangqamuzana esingaba ngu-13.7 kDa.I-RNase A i-endoribonuclease eyehlisa ngokukhethekile i-RNA enomucu owodwa ezinsaleni zika-C no-U.Ngokuqondile, i-cleavage ibona isibopho se-phosphodiester esakhiwe yi-5'-ribose ye-nucleotide kanye neqembu le-phosphate ku-3'-ribose ye-pyrimidine nucleotide eseduze, ukuze i-2', 3' - i-cyclic phosphates i-hydrolyzed ehambisanayo. I-3'-nucleoside phosphates (isb, i-pG-pG-pC-pA-pG ihlukaniswa yi-RNase A ukuze kukhiqizwe i-pG-pG-pCp kanye ne-A-PG).I-RNase A iyona esebenza kakhulu ekuhlukaniseni i-RNA enomucu owodwa futhi isebenza ezimeni ezihlukahlukene zokusabela: ekugxilweni kukasawoti ophansi (0 kuya ku-100 mM NaCl), ingasetshenziswa ukuhlukanisa i-RNA enomucu owodwa, i-RNA ephindwe kabili, kanye nemicu ye-RNA kuma-RNA-DNA hybrids.kuyilapho isezingeni eliphezulu likasawoti (≥0.3 M), i-RNase A ingahlukanisa ngokukhethekile i-RNA enomucu owodwa.