I-Proteinase K(Uketshezi)
Izinzuzo
● Ukuzinza okuphezulu nomsebenzi we-enzyme osuselwe kubuchwepheshe obuqondisiwe bokuziphendukela kwemvelo
● Iyawubekezelela usawoti we-Guanidine
● I-RNase mahhala, i-DNase imahhala ne-Nickase mahhala, i-DNA <5 pg/mg
Incazelo
I-Proteinase K iyi-serine protease ezinzile enokucaciswa okubanzi kwe-substrate.Yehlisa isithunzi samaprotheni amaningi endaweni yendabuko ngisho nalapho kukhona izinto zokuhlanza.Ubufakazi obuvela ocwaningweni lwekristalu nesakhiwo samangqamuzana bubonisa ukuthi i-enzyme iyingxenye yomndeni we-subtilisin ene-site catalytic triad esebenzayo (Asp 39-His 69-Ser 224).Indawo ephambili yokuqhekeka isibopho se-peptide esincikene neqembu le-carboxyl lama-amino acid aliphatic kanye nephunga elimnandi elinamaqembu e-alpha amino avinjiwe.Ngokuvamile isetshenziselwa ukucaciswa kwayo okubanzi.
Isakhiwo samakhemikhali
Ukucaciswa
| Izinto zokuhlola | Imininingwane |
| Ukubukeka(Umbala) | Ayinambala ukuya ku-Light Brown |
| Ukubukeka( Turbidity) | Kucace Kuya Kufiphele Kancane Kakhulu |
| Ukubukeka( Ifomu) | Uketshezi |
| Umsebenzi | ≥800U/ml |
| Amaprotheni mg / ml | ≥20 mg/ml |
| RNase | Akukho okutholiwe |
| DNase | Akukho okutholiwe |
| Nicase | Akukho okutholiwe |
Izinhlelo zokusebenza
Ikhithi yokuxilonga yofuzo;
I-RNA kanye ne-DNA extraction kits;
Ukukhishwa kwezingxenye ezingezona amaprotheni ezicutshini, ukuwohloka kokungcola kwamaprotheni, njenge
Imithi yokugoma ye-DNA kanye nokulungiswa kwe-heparin;
Ukulungiswa kwe-chromosome DNA nge-pulsed electrophoresis;
Ibala laseNtshonalanga;
Ama-enzymatic glycosylated albumin reagents in vitro diagnostics
Ukuthumela kanye Nesitoreji
Ukuthumela:I-ambient
Imibandela Yesitoreji:Gcina ku -20℃(Isikhathi eside)/ 2-8℃(Isikhathi esifushane)
Idethi yokuhlola kabusha enconyiwe:iminyaka engu-2
