I-Proteinase K(Lyophiled Powder)
Izinzuzo
● Ukuzinza okuphezulu nomsebenzi we-enzyme osuselwe kubuchwepheshe obuqondisiwe bokuziphendukela kwemvelo
● Iyawubekezelela usawoti we-Guanidine
● I-RNase mahhala, i-DNase imahhala ne-Nickase mahhala, i-DNA <5 pg/mg
Incazelo
I-Proteinase K iyi-serine protease ezinzile enokucaciswa okubanzi kwe-substrate.Yehlisa isithunzi samaprotheni amaningi endaweni yendabuko ngisho nalapho kukhona izinto zokuhlanza.Ubufakazi obuvela ocwaningweni lwekristalu nesakhiwo samangqamuzana bubonisa ukuthi i-enzyme iyingxenye yomndeni we-subtilisin ene-site catalytic triad esebenzayo (Asp 39-His 69-Ser 224).Indawo ephambili yokuqhekeka isibopho se-peptide esincikene neqembu le-carboxyl lama-amino acid aliphatic kanye nephunga elimnandi elinamaqembu e-alpha amino avinjiwe.Ngokuvamile isetshenziselwa ukucaciswa kwayo okubanzi.
Isakhiwo samakhemikhali
Ukucaciswa
| Izinto zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe ukuya emhlophe, i-Lyophilied |
| Umsebenzi | ≥30U/mg |
| I-Solubility(50mg Powder/mL) | Kucace |
| RNase | Akukho okutholiwe |
| DNase | Akukho okutholiwe |
| I-Nickase | Akukho okutholiwe |
Izinhlelo zokusebenza
Ikhithi yokuxilonga yofuzo;
I-RNA kanye ne-DNA extraction kits;
Ukukhishwa kwezingxenye ezingezona amaprotheni ezicutshini, ukuwohloka kokungcola kwamaprotheni, njenge
Imithi yokugoma ye-DNA kanye nokulungiswa kwe-heparin;
Ukulungiswa kwe-chromosome DNA nge-pulsed electrophoresis;
Ibala laseNtshonalanga;
Ama-enzymatic glycosylated albumin reagents in vitro diagnostics
Ukuthumela kanye Nesitoreji
Ukuthumela:I-ambient
Imibandela Yesitoreji:Gcina ku -20℃(Isikhathi eside)/ 2-8℃(Isikhathi esifushane)
Idethi yokuhlola kabusha enconyiwe:iminyaka engu-2
Izinyathelo zokuzivikela
Gqoka amagilavu okuzivikela kanye nezibuko lapho usebenzisa noma ukala, futhi uhlale unomoya omuhle ngemva kokuwusebenzisa.Lo mkhiqizo ungase ubangele ukungezwani nesikhumba.Banga ukucasuka kwamehlo okukhulu.Uma uhogele, kungase kubangele ukungezwani komzimba noma izimpawu zesifuba somoya noma i-dyspnea.Ingabangela ukucasuka kokuphefumula.
I-Assay Unit Definition
Iyunithi eyodwa (U) ichazwa njengenani le-enzyme edingekayo ukuze i-hydrolyze i-casein ukukhiqiza i-1 μmol tyrosine ngomzuzu ngaphansi kwezimo ezilandelayo.
