Isithombe Esifakiwe Sokukhawulelwa kwe-BsaI Endonuclease

I-BsaI Restriction Endonuclease

Ubumsulwa: 20U/μL

Iphakheji: 50μL, 250μL, 1ml, 10ml, 100ml

Thumela Uphenyo

Imininingwane Yomkhiqizo

Incazelo

I-enzyme evimbela i-BsaI iwuhlobo lwe-endonuclease evimbelayo yohlobo lwe-II etholakala kuphrotheni ehlanganisiwe efakwe isakhi sofuzo se-BspQI ku-Bacillus sphaericus evezwa i-E.coli.Ama-endonuclease okuvimbela asetshenziswa kakhulu ekwenzeni imephu yezakhi zofuzo, i-cloning nezinye izinkambu.Le enzyme isika ngokushesha i-DNA ukuze isetshenziswe ngempumelelo isakhi sofuzo i-Bsa I , i-endonuclease restriction endonuclease restriction IIs, itholakala ohlotsheni oluhlangene lwe-E. coli oluthwala isakhi sofuzo se-BsaI esihlanganisiwe nesishintshiwe esivela ku-Bacillus stearothermophilus.ezinokucaciswa okufanayo njengama-enzyme omdabu kanye nomsebenzi wenkanyezi oncishisiwe.Ukulandelana kwayo okuqashelwayo kanye nezingosi zokuhlukanisa zimi kanje:

5'······GGTC(N)········3'

3'······CCAGAG(NNNNN)······5'

Isakhiwo Samakhemikhali

Izici zomkhiqizo

• I-endonuclease ekhawulelwe inomsebenzi ophezulu we-enzymatic futhi iqeda ukusabela kokugaya ngokushesha.

• Umsebenzi ophansi we-endonuclease ongaqondile ukuqinisekisa ukusika okunembile "kwe-scalpel".

• Alukho uhlelo lwe-BSA, olunciphisa ukungcoliswa komthombo wokushisa.

Ukucaciswa

Izinto Zokuhlola	Imininingwane
Umsebenzi we-Enzyme	20 U/µL
Ubumsulwa(SDS-PAGE)	≥95%
I-Rnase	Akukho okutholiwe
Umsebenzi we-DNase ongaqondile	Akutholwanga
Inkanyezi Umsebenzi	Akutholwanga
Ligation kanye Recutting	Ngemva kokugaya, izingcezu ze-DNA zingahlanganiswa.Futhi lezi zingcezu ezinezintambo zingasikwa kabusha nge-Bsal.
I-Endotoxins(LAL-test)	≤10 EU/mg
E.Coli DNA	≤1 Ikhophi/2U

Ikhwalithi yokulawula

Ubumsulwa ≥ 95%, Ayikho i-DNase, umsebenzi we-RNase, insalela ye-DNA Yokusingatha ≤ 100pg/mg, insalela yamaprotheni abamba ≤ -50ppm, insalela ye-endotoxin ≤ 10EU/mg, Awukho umsebenzi we-protease, oyinyumba, awunayo i-mycoplasma

Isicelo

Gaya i-plasmid ukuze ulungise ucezu lwe-DNA olunomugqa ekugcineni kwe-Poly (A/T/C/G);

Yenza umugqa wesifanekiso se-plasmid ngaphambi kokulotshwa kwe-in vitro;

Inhlabamkhosi ekhawulelwe;

Incazelo Yeyunithi

Iyunithi eyodwa ichazwa njengenani le-enzyme edingekayo ukuze kugaywe i-1 µg ye-DNA yokulawula kwangaphakathi ngehora elingu-1 ku-37°C kuvolumu yokusabela ephelele engu-50 µL.