I-Trypsin ehlanganisiwe
Inzuzo
1.Ayikho imvelaphi yezilwane
2.Ukuhlanzeka okuphezulu
3.Ukulawula inqubo engcono
4.Izinga elizinzile
Incazelo
I-Trypsin ihlukanisa ngokuqondile amabhondi e-C-terminal peptide e-lysine ne-arginine, angehlisa isithunzi samaprotheni ahlanganisa ama-intercellular.Ukulandelana kwe-amino acid ye-trypsin ephinde yahlanganiswa ekhiqizwa yi-Hzymes Biotech kuyafana naleyo ye-trypsin etholakala kumapancreas, futhi ikhiqizwa i-recombinant Escherichia coli expression.Ingangena esikhundleni sokukhishwa kwendabuko kwe-trypsin ukuze kugaywe amangqamuzana emithini yokugoma, ama-stem cells, ukwelashwa kwamaseli omzimba, ukuhlolwa kwezidakamizwa, amasosha omzimba kanye neminye imikhakha.I-Aprotinin, i-soybean trypsin inhibitor, njll. ingavimbela ngokusobala umsebenzi we-trypsin.
I-recombinant trypsin inezinto ezifanayo ne-trypsin ekhishwe ngokwemvelo.Lo mkhiqizo ukhiqizwa endaweni yokusebenzela yebanga le-GMP.Azikho izinto zokusetshenziswa zemvelaphi yezilwane ezisetshenziswa ekuvubeleni, ekuhlanzweni nasezithakweni zokugcina.Ngesikhathi esifanayo, izinqubo zokukhiqiza nekhwalithi ziqinisekisiwe, okuholela ezingeni eliphezulu lokungaguquguquki phakathi kwamaqoqo.
Isakhiwo Samakhemikhali
Incazelo Yeyunithi
25℃, pH 7.6, 3.2ml reaction solution(1cm light path), iyunithi eyodwa ye-trypsin (U) yachazwa ngokuthi
ukwanda ngo-0.003 enanini lokumuncwa ku-253nm nge-enzymatic hydrolysis ye-BAEE ngomzuzu.
Ukusetshenziswa komkhiqizo
Ukuze isiko cell
• Ukugaya kwezicubu, ukutholwa kwamaseli okuyisisekelo.
• Ukudlula kokugaya kwamaseli anamathelayo.
• Isiko lamaseli ngendlela ye-microcarrier.
• Ukugaya kancane ama-stem cells.
• Ukwelashwa kwamangqamuzana amasosha omzimba, njll.
Okwephrotheni ehlanganisiwe
• Ukukhiqizwa kabusha kwe-insulin.
• Ukulandelana kwamaprotheni, imephu ye-peptide.
• Izinqubo ezithile ze-proteolytic ezifana nocwaningo lwe-proteomics
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Ukubukeka | Impushana emhlophe noma evaliwe |
| Ukunyibilika (10mg/ml) | Sula isixazululo esingenambala |
| Ubumsulwa | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Umsebenzi othize(Powder) | ≥2500U/mg |
| I-E.Coli ibamba izinsalela zamaphrotheni | ≤0.01% |
| I-Endotoxins(LAL-Test) | ≤20 EU/mg |
Ezokuthutha nokugcina
Ezokuthutha:Kuthunyelwa ngaphansi kuka-2-8 °C
Isitoreji:Gcina ku-2-8°C, ngemva kokuncibilikiswa ne-1mM hcl noma 50nM HAC, kufanele igcinwe ngaphansi -25~-15 °C
Impilo yokuhlola kabusha enconyiwe:2 iminyaka
