I-Deoxyribonuclease I(Dnase I)

I-DNase I (Deoxyribonuclease I) iyi-endodeoxyribonuclease engagaya i-DNA enemicu eyodwa noma emibili.Ibona futhi ihlukanise amabhondi e-phosphodiester ukuze kukhiqizwe i-monodeoxynucleotides noma i-oligodeoxynucleotides enemicu eyodwa noma ekabili namaqembu e-phosphate ku-5'-terminal kanye ne-hydroxyl ku-3'-terminal.Umsebenzi we-DNase I uncike ku-Ca 2+ futhi ungenziwa isebenze ngama-ion ensimbi e-divalent njenge-Mn 2+ ne-Zn 2+.I-5 mM Ca 2+ ivikela i-enzyme ku-hydrolysis.Lapho kukhona i-Mg 2+, i-enzyme yayikwazi ukubona ngokuzenzakalelayo futhi ihlukanise noma iyiphi indawo kunoma yiluphi umucu we-DNA.Ebukhoneni be-Mn 2+, imicu ephindwe kabili ye-DNA ingaqashelwa kanyekanye futhi iqhekezwe cishe endaweni efanayo ukuze kwakheke izingcezu ze-DNA eziyisiphetho esiyisicaba noma izingcezu ze-DNA ezinamathelayo ezinama-nucleotide angu-1-2 aphumela ngaphandle.