I-dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM)
Incazelo
Lo mkhiqizo uyisixazululo samanzi esingenambala.Ilungele ukuhlola okujwayelekile kwebhayoloji yamangqamuzana okufana nokukhulisa i-PCR, i-PCR yesikhathi sangempela, i-cDNA noma ukuhlanganiswa kwe-DNA okuvamile, ukulandelana kwe-DNA nokulebula.Ingahlanjululwa ngamanzi ahlanzekile kakhulu, futhi ilungiswe ku-pH 7.0 ngesisombululo se-NaOH esihlanzekile, ngobumsulwa ≥ 99% (HPLC).Ngemva kokutholwa, ayiqukethe i-DNase, i-RNase ne-phosphotase.Ingasetshenziswa ngokuqondile ekuphenduleni okujwayelekile kwamangqamuzana ezinto eziphilayo njenge-PCR.
Isakhiwo samakhemikhali
Ukucaciswa
| Izinto zokuhlola | Imininingwane |
| Ukubukeka | Sula isixazululo esingenambala |
| Ukugxila | 100mM ±3% |
| Ubumsulwa (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Ukulawulwa Kwekhwalithi1.Lokhu kulungiselelwa akukho ekungcoleni kwe-DNase ne-RNase 2. Le dNTP eningi ihlolwe ukusebenza nge-Taq DNA polymerase kanye ne-Pfu DNA polymerase. Le ngxenye ye-d NTP ihlolwe ukusebenza nge-Q-PCR | |
Izinhlelo zokusebenza
I-PCR yebanga elide (20 KB)
I-cDNA synthesis kanye ne-RT-PCR
I-PCR yesikhathi sangempela
I-PCR ejwayelekile
Inani eliphakeme kakhulu lama-PCR
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -25～-15℃
Idethi yokuhlola kabusha enconyiwe:iminyaka engu-2
Amanothi
1) Ingancibilika ekamelweni lokushisa.Ngemuva kokuqedwa, kufanele igcinwe ebhokisini leqhwa noma i-ice bath.Ngemuva kokusetshenziswa, kufanele igcinwe ku -25～-15 ℃ ngokushesha.
2) Ukuze uphephe kanye nempilo yakho, sicela ugqoke amajazi elebhu namagilavu alahlwayo ukuze usebenze.
