Multiplex PCR Taq DNA Polymerase

I-Multiplex PCR DNA Polymerase isizukulwane esisha se-DNA polymerase eqala ukushisa esekelwe ekuguqulweni kwe-antibody futhi yathuthukiswa ukuze kuthuthukiswe ukuhlobana kwesifanekiso.Inguqulo ye-High sensitivity yenzelwe ngokucophelela i-multiplex PCR enekhono eliqinile lokukhulisa nokuzwela okuphezulu.Ngohlelo olulungile lwebhafa lwe-multiplex PCR, luhambisana nohlu olubanzi lokuqukethwe komkhiqizo we-GC kanye namanani okuqala we-Tm.Iningi lama-multiplex PCR assays angenziwa ezingeni lokushisa elivamile le-annealing elingu-60°C ngaphandle kokulungiselelwa okwengeziwe.

Lo mkhiqizo unekhono eliphezulu kakhulu lokukhulisa i-amplification, futhi ungakhulisa kanyekanye izingcezu eziqondiwe ebangeni lika-50 - 3,500 bp;inekhono eliqinile lokukhulisa i-amplification, futhi ingakwazi ukucebisa amakhulu ambalwa noma ngaphezulu kokukhulisa;ukubekezelelana okuhle kakhulu kokungcola, ukumelana kakhulu nezinhlobonhlobo zama-inhibitor nezinhlobo zokungcola, futhi kuhambisana nokukhuliswa okuqondile kwegazi lonke, amakhadi egazi, njll. Ama-reagents anokuqina okuhle, ukusebenza okubanzi, futhi ahambisana nezimo ezihlukahlukene zokutholwa.