I-Uracil DNA Glycosylase (UDG/UNG)
Incazelo
I-Heat-Labile UDG (uracil DNA glycosylase) igqugquzela ukukhululwa kwe-uracil yamahhala ku-DNA equkethe i-uracil ngokwenza i-hydrolyzing isibopho se-N-glycoside phakathi kwezisekelo ze-uracil kanye namathambo e-sugar-phosphate skeleton.Uma kuqhathaniswa nama-enzyme avamile e-UDG, i-heat-Labile UDG ingagwema ukucekelwa phansi kwemikhiqizo yokukhulisa equkethe i-dU ebangelwa umsebenzi osele we-UDG engasebenzi ekamelweni lokushisa.I-Heat-Labile UDG (uracil DNA glycosylase) isebenza ekamelweni lokushisa futhi i-thermolabile futhi kulula ukuyivala.
Isakhiwo Samakhemikhali
Ukucaciswa
| I-enzyme | I-Glycosylase |
| Ibhafa Ehambisanayo | Ibhafa Yesitoreji |
| Ukushisa Ukungasebenzi | 50°C, imizuzu eyi-10 |
| Incazelo Yeyunithi | Iyunithi eyodwa (U) ichazwa njengenani le-enzyme edingekayo ukuze kusungulwe i-hydrolysis ye-1 μg dU equkethe i-dsDNA ngemizuzu engama-30 ku-25°C. |
Izinhlelo zokusebenza
1.Ucwaningo lokulungiswa kwe-DNA nokutholwa kokuguqulwa kwezakhi zofuzo.
2.Khulisa ukusebenza kahle kwe-cloning kwemikhiqizo ye-PCR.
3.Ukwandisa ukusebenza kahle kwe-mutagenesis eqondiswe kusayithi.
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -15℃ ~ -25℃
Impilo ye-Shief:iminyaka engu-2
