I-DNase I (Rnase Free)(5u/ul) HC4007A Isithombe Esifakiwe

I-DNase I (I-Rnase Free)(5u/ul)

Inombolo yekati: HC4007A

Iphakheji: 1000U/5000U/50000U

I-DNase I (Deoxyribonuclease I) iyi-endodeoxyribonuclease engagaya i-DNA enemicu eyodwa noma ekabili.

Thumela Uphenyo

Incazelo Yomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati: HC4007A

I-DNase I (Deoxyribonuclease I) iyi-endodeoxyribonuclease engagaya i-DNA enemicu eyodwa noma ekabili.Ibona futhi ihlukanise amabhondi e-phosphodiester ukuze kukhiqizwe i-monodeoxynucleotides noma i-oligodeoxynucleotides enemicu eyodwa noma kabili namaqembu e-phosphate ku-5'-terminal kanye ne-hydroxyl ku-3'-terminal.Umsebenzi we-DNase I uncike ku-Ca²⁺futhi ingenziwa isebenze ngama-ion ensimbi e-divalent afana noMn²⁺kanye neZn²⁺.5 mM Ca²⁺ivikela i-enzyme ku-hydrolysis.Phambi kukaMg²⁺, le enzyme ingabona noma ihlukanise noma iyiphi indawo kunoma yimuphi umucu we-DNA.Phambi kukaMnu²⁺, imicu ekabili ye-DNA ingaqashelwa kanyekanye futhi iqhekeke endaweni efanayo ukuze yakhe izingcezu ze-DNA eziyisicaba noma izingcezu ezinamathelayo ze-DNA ezinamanucleotide angu-1-2 aphumela ngaphandle.

Okwedlule: I-Rnase A HC3905B

Olandelayo: I-DNase I (Rnase Free) (2u/ul) HC4007B

Izingxenye

Igama	0.1KU	1KU	5 KU	50 KU
DNase I, RNase-free	20μL	200μL	1mL	10 ml
10×DNase I Buffer	1mL	1mL	5 × 1mL	5 × 10 mL

Izimo zokugcina

-25 ℃~-15 ℃ yokugcina;Ezokuthutha ngaphansi kwamaphakethe eqhwa.

Iziyalezo

1. Lungiselela isixazululo sokusabela kushubhu elingenayo i-RNase ngokwezilinganiso ezibalwe ngezansi:

Isakhi	Ivolumu
I-RNA	Xµg
10 × DNase I Buffer	1μL
I-DNase I, i-RNase-free(5U/μL)	1 U nge-µg RNA①
ddH₂O	Kufika ku-10μL

Qaphela: ①Bala ivolumu ye-DNase I okudingeka ingezwe ngokusekelwe enanini le-RNA.

2. 37 ℃ imizuzu engu-15;

3. Engeza u-0.5M EDTA ekuhlanganiseni kokugcina kuka-2.5mM~5mM, bese ushisisa ku-65℃ imizuzu engu-10 ukuze umise ukusabela.Isampula ingasetshenziswa ngokuqondile ekuphenduleni okulandelayo njengokulotshwa okuhlanekezelweukuhlola.

Incazelo Yeyunithi

Iyunithi eyodwa ichazwa njengenani le-enzyme elizokwehlisa ngokuphelele i-1µg ye-pBR322I-DNA emizuzwini eyi-10 ku-37 ℃.

Ikhwalithi yokulawula

I-RNase:I-5U ye-DNase I ene-1.6µg MS2 RNA amahora angu-4 ku-37℃ ayikhiqizi njengobakunqunywa i-agarose gel electrophoresis.

Amanothi

1. Sicela ulungiselele i-0.5MEDTA uwedwa.

2. Sebenzisa i-1U DNase I nge-µg ye-RNA.Nokho, uma i-RNA ingaphansi kuka-1µg, sicela usebenzise i-1U DNase I.

3. Sicela ubeke i-enzyme eqhweni ngesikhathi sokusebenza.