I-Recombinant Deoxyribonuclease I (DNase I, RNase-free)
Incazelo
I-Recombinant Deoxyribonuclease I, i-endonuclease engagaya i-DNA enomucu owodwa noma enemicu ekabili.I-hydrolyzes phosphodiester bond ukuze ikhiqize i-mono- ne-oligodeoxynucleotides equkethe amaqembu angu-5'-phosphate kanye namaqembu angu-3'-OH.Ububanzi obulungile be-pH be-DNase I bungu-7-8.Umsebenzi we-DNase I uncike ku-Ca2+ futhi ungenziwa isebenze ngama-ion ensimbi e-divalent afana ne-Co2+, Mn2+, Zn2+, njll. Ebukhoneni be-Mg2+, DNase Ngingakwazi ukusika ngokungahleliwe i-DNA enemicu ekabili kunoma iyiphi indawo;kuyilapho ebukhoneni be-Mn2+, i-DNase I ngikwazi ukusika i-DNA ephindwe kabili endaweni efanayo, yenze iziphetho ezibuthuntu noma ama-nuclei angu-1-2 Anamathelayo aphethe ngokumbondela kwe-nucleotide.Le enzyme itholakala ezinhlobonhlobo ze-E. coli, ayiqukethe i-RNAse, futhi ingasetshenziselwa ukucubungula amasampula ahlukahlukene e-RNA.
Isakhiwo samakhemikhali
Izinhlelo zokusebenza
Isizinda se-RNA: lungiselela i-RNA ngaphandle kwe-DNA;
Ngemva kokubhalwa kwe-in vitro nge-RNA polymerase, njenge-T7 RNA Polymerase (Cat#10618), isetshenziselwa ukususa isifanekiso se-DNA;
Lungiselela i-RNA engena-DNA ngaphambi kwe-RT-PCR ne-RT-qPCR;
Isetshenziswa ngokuhambisana ne-DNA Polymerase I (Cat#12903) yokulebula i-DNA ngokuhumusha kwesiteketiso;
Isetshenziswa ekuhlaziyeni kwezinyawo ukuze kuhlaziywe ukusebenzisana kwe-DNA-amaprotheni;
Khiqiza umtapo wolwazi wezingcezu ezingahleliwe;
Ekutholweni kwe-TUNEL ye-apoptosis, i-DNA ye-genomic eshebe kancane yasetshenziswa njengokulawula okuhle.
Ukuthumela kanye Nesitoreji
Ezokuthutha:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -20 ℃
Impilo Yeshelufu:iminyaka engu-2
Amanothi:
1.Ama-Enzyme kufanele agcinwe ebhokisini leqhwa noma endaweni yokugeza yeqhwa lapho esetshenziswa, futhi kufanele agcinwe ku -20°C ngokushesha ngemva kokusetshenziswa.
2.Ukuze uphephe nempilo yakho, sicela ugqoke izinto zokuzivikela (i-PPE), njengamajazi aselabhorethri namagilavu alahlwayo, lapho usebenza nalo mkhiqizo.
