I-AMPPD-Chemiluminescent Substrate
Incazelo
I-AMPPD inezingxenye ezimbili ezibalulekile esakhiweni sayo samangqamuzana.Enye i-dioxygen
indandatho ye-tetrad ehlanganisa iringi ye-benzene ne-adamantane, engaphuka futhi ikhiphe ama-photon.Okunye ukuba khona kwe-alkaline phosphatase, eyenza ukukhishwa kwamaqembu e-phosphate futhi kwakha okuphakathi okungazinzile.I-intermediate yabe isibola ngokuzenzekelayo futhi yakhipha ama-photon, futhi inani lama-photon akhululwa lalilingana nokugxiliswa kwe-ALP esixazululweni, okwakufanele uhlelo lwe-chemiluminescence immunoassay olune-ALP njenge-enzyme ebizwa ngokuthi.
Sebenzisa:
I-AMPPD iwuketshezi lwe-chemiluminescent olunokuzwela okuphezulu, ukuzinza kanye nesilinganiso sesignali-kuya-noise, olungase lusetshenziselwe ukutholwa kokuzivikela komzimba kwe-Alkaline Phosphatase (ALP) njengomaka.
Isakhiwo Samakhemikhali
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Uketshezi oluhlanzekile oluphuzi oluluhlaza |
| Ukuzinza | Inani le-Iuminescence lika-37℃ lagcinwa lingaphezulu kwama-90% ngemva kwezinsuku eziyi-7 zokufukamela ngaphansi kokuvikelwa kokukhanya. |
| Inani le-pH | 9.5±0.05 |
| Inani langemuva | 200-10000 |
| I-Luminecence value ephezulu ye-CV value (uma iqhathaniswa nendinganiso, izimo zokusabela zaziyi-150+5μL, futhi ukuhlushwa kwe-enzyme kwakungu-0.0032U/mL). | ≤10% |
Ezokuthutha nokugcina
Ezokuthutha:I-ambient
Isitoreji :Gcina ku-2-8 ° C, uvikeleke ngokuqinile ekukhanyeni
Impilo yokuhlola kabusha enconyiwe:2 iminyaka
