I-D-Lactate dehydrogenase(LDH)
Incazelo
I-enzyme isetshenziselwa ukususwa kwe-pyruvate ekunqumeni okusebenzisana ne-NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Ukusetshenziswa
I-1.I-alkaline phosphatase ehlanganiswe namaprotheni (amasosha omzimba, i-streptavidin njll,) ingakwazi ukubona ngokuqondile ama-molecule aqondiwe, futhi ingasetshenziswa ku-ELISA, WB kanye nokutholwa kwe-histochemical;
2.I-alkaline phosphatase ingasetshenziswa ukwenza i-dephosphorize i-5 '-terminal ye-DNA noma i-RNA ukuvimbela ukuzixhumanisa;
3.I-DNA ye-dephosphorylated engenhla noma i-RNA ingalebula ngama-phosphates abhalwe umsakazo (nge-T4 poly-nucleotide kinase)
Isakhiwo Samakhemikhali
Indlela yokusabela
I-D-Lactate + NAD+→ I-Pyruvate +NADH + H+
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe e-amorphous, i-lyophilized |
| Umsebenzi | ≥200U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg powder/mL) | Kucace |
| I-NADH/NADPH oxidase | ≤0.01% |
| I-Malate dehydrogenase | ≤0.005% |
| I-Glutamate dehydrogenase | ≤0.003% |
| I-Pyruvate kinase | ≤0.03% |
| I-Glutamate Dehydrogenase | ≤0.003% |
| I-Aspartate aminotransferase | ≤0.001% |
| I-Alamine aminotransferase | ≤0.001% |
Ezokuthutha nokugcina
Ezokuthutha:I-AmbIent
Isitoreji :Gcina ku -20°C(Isikhathi eside), 2-8°C (isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo:2 iminyaka
