I-Glucose-6-phosphate dehydrogenase (G6PDH)
Incazelo
Ukushoda kwe-Glucose 6 phosphate dehydrogenase (G6PD) kuyisimo sofuzo lapho amangqamuzana egazi abomvu ephuka khona (i-hemolysis) lapho umzimba ubhekene nokudla okuthile, izidakamizwa, izifo noma ingcindezi.Kwenzeka lapho umuntu engekho noma enamazinga aphansi e-enzyme glucose-6-phosphate dehydrogenase.Le enzyme isiza amangqamuzana egazi abomvu asebenze kahle.Izimpawu phakathi nesiqephu se-hemolytic zingabandakanya umchamo omnyama, ukukhathala, ukuphaphatheka, ukushaya kwenhliziyo okusheshayo, ukuphefumula, nokuphuzi kwesikhumba (i-jaundice).Ukushoda kwe-G6PD kuzuzwa njengefa ngendlela exhunywe ukuphindaphindeka exhunywe ku-X futhi izimpawu zivame kakhulu kwabesilisa (ikakhulukazi abantu base-Afrika baseMelika kanye nalabo abavela ezindaweni ezithile zase-Afrika, e-Asia, naseMedithera).Kubangelwa izinguquko zofuzo kufuzo lwe-G6PD.
I-Glucose-6-Phophate Dehydrogenase (G-6-PDH) iqukethe amayunithi amabili esisindo sengqamuzana esilinganayo.Ukulandelana kwe-amino acid ye-monomer kushicilelwe. ama-nucleotides.I-G-6-PDH ingaphinda isebenze kusukela kuzixazululo ze-urea-denatured.
I-Glucose 6-phosphate dehydrogenase iyi-enzyme eyinhloko yokulawula esinyathelweni sokuqala se-pentose phosphate pathway.I-G-6-P-DH i-oxidize i-glucose-6-phosphate phambi kwe-NADP+ ukukhiqiza i-6- phosphogluconate.I-Polyacrylamide gel electrophoresis, ukungcolisa umsebenzi, kanye nezifundo ze-anti-yeast ye-G-6-PDH zokuvimba amasosha omzimba ziye zabonisa ukuthi i-G-6-PDH iyi-glycoprotein.
Isakhiwo Samakhemikhali
Isimiso Sokusabela
I-D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe e-amorphous, i-lyophilized |
| Umsebenzi | ≥150U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg powder/ml) | Kucace |
| I-NADH/NADPH oxidase | ≤0.1% |
| I-Glutathione reductase | ≤0.001% |
| I-Phosphoglucose isomerase | ≤0.001% |
| I-Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| I-Myokinase | ≤0.01% |
| I-Hexokinase | ≤0.001% |
Ezokuthutha nokugcina
Ezokuthutha: I-ambient
Isitoreji :Gcina ku-2-8°C
Ukuhlola kabusha okunconyiweImpilo:2 iminyaka
