I-Phosphatase Alkaline (ALP)

Inombolo yeCas :9001-78-9

EC No.:3.1.3.1

Iphakheji: 100μL, 500μL, 10mL, 100mL, 1000mL

Thumela Uphenyo

Incazelo Yomkhiqizo

Incazelo

I-Alkaline Phosphatase isuselwa ohlotsheni oluhlanganisiwe lwe-E. coli oluthwala isakhi sofuzo se-TAB5.I-enzyme ivuselela i-dephosphorylation ye-5' kanye ne-3' ekupheleni kwe-DNA kanye ne-RNA phosphomonoesters.Futhi, i-hydrolyses i-ribose, kanye ne-deoxyribonucleoside triphosphates (NTPs kanye nama-dNTPs).I-TAB5 i-Alkaline Phosphatase isebenza ekuphumeni okungu-5, oku-5' kokuhlehla kanye neziphetho ezibuthuntu.I-Phosphatase ingasetshenziswa ezinhlelweni eziningi zebhayoloji yamangqamuzana, njengokuhlanganisa i-cloning noma ukulebula kokuphela kwe-probe ukuze kukhishwe iziphetho ezine-phosphorylated ze-DNA noma i-RNA.Ekuhlolweni kwe-cloning, i-dephosphorylation ivimbela i-DNA ye-plasmid eqondile ekuzibopheni.Ingase futhi yehlise izinga le-dNTP engahlanganisiwe ekuphenduleni kwe-PCR ukuze ilungiselele isifanekiso sokulandelana kwe-DNA.I-enzyme yenziwe ingasebenzi ngokuphelele futhi ngokungenakuhlehliswa ngokushisisa ku-70°C imizuzu emi-5, ngaleyo ndlela yenze ukususwa kwe-phosphatase ngaphambi kokuxhunywa noma ukuqeda ukulebula kungadingekile.

Ukusetshenziswa

I-1.I-Alkaline phosphatase ehlanganiswe namaprotheni (amasosha omzimba, i-streptavidin njll,) ingakwazi ukubona ngokuqondile ama-molecule aqondiwe, futhi ingasetshenziswa ku-ELISA, WB kanye nokutholwa kwe-histochemical;
2.I-alkaline phosphatase ingasetshenziswa ukwenza i-dephosphorize i-5 '-terminal ye-DNA noma i-RNA ukuvimbela ukuzixhumanisa;
3.I-DNA ye-dephosphorylated engenhla noma i-RNA ingalebula ngama-phosphates abhalwe umsakazo (nge-T4 poly-nucleotide kinase)

Isakhiwo Samakhemikhali

Ukucaciswa

Izinto Zokuhlola	Imininingwane
Umsebenzi we-Enzyme	5U/μL
Umsebenzi we-Endonuclease	Ayitholakali
Umsebenzi we-Exonuclease	Ayitholakali
Umsebenzi Wokudlala	Ayitholakali
Umsebenzi we-RNase	Ayitholakali
E.coli DNA	≤1ikhophi/5U
I-Endotoxin	I-LAL-Test, ≤10EU/mg
Ubumsulwa	≥95%