I-Hexokinase (HK)
Incazelo
Sebenzisa i-Hexokinase ukuze uthole i-D-glucose, D-fructose, ne-D-sorbitol ekudleni noma kumasampula ocwaningo lwebhayoloji.I-enzyme iphinde isetshenziselwe ukuhlolwa kwamanye ama-saccharides aguqulwa abe yi-glucose noma i-fructose, ngakho-ke iwusizo ekuhlolweni kwama-glycosides amaningi.
Uma i-Hexokinase isetshenziswa ngokuhlanganiswa ne-glucose-6-phosphate dehydrogenase (G6P-DH)* (izivivinyo ze-glucose6-phosphate ezakhiwe yi-Hexokinase), amasampula akufanele abe nokugxila okuphezulu kwe-phosphate njengoba i-G6P-DH ivinjwa ngokuncintisana yi-phosphate.
Isakhiwo Samakhemikhali
Isimiso Sokusabela
I-D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Okumhlophe kuya kokuphuzi okuncane i-amorphous powder, i-lyophilized |
| Umsebenzi | ≥30U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg powder/ml) | Kucace |
| AmaProtease | ≤0.01% |
| I-ATPase | ≤0.03% |
| I-Phosphoglucose isomerase | ≤0.001% |
| I-Creatine phosphokinase | ≤0.001% |
| IGlucose-6-Phosphate dehydrogenase | ≤0.01% |
| I-NADH/NADPH oxidase | ≤0.01% |
Ezokuthutha nokugcina
Ezokuthutha: Ai-mbient
Isitoreji :Gcina ku -20°C(Isikhathi eside), 2-8°C (isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo:1 unyaka
