I-Glycerol Kinase(GK)
Incazelo
Iphrotheni efakwe yileli sakhi sofuzo ingeyomndeni we-FGGY kinase.Le phrotheni iyi-enzyme eyinhloko ekulawulweni kokuthathwa kwe-glycerol kanye ne-metabolism.Ikhuthaza i-phosphorylation ye-glycerol nge-ATP, ikhiqiza i-ADP ne-glycerol-3-phosphate.Ukuguqulwa kwalesi sakhi sofuzo kuhlotshaniswa nokuntuleka kwe-glycerol kinase (GKD).Okunye okuhlukile kokulotshiweyo okuhlukene okuhlanganisa ama-isoform ahlukene kutholakalele lolu fuzo.
Le enzyme isetshenziselwa ukuhlolwa kokuxilonga ukuze kutholwe ama-triglycerides kanye ne-Glycerol-3-phosphate Oxidase.
Isakhiwo Samakhemikhali
Isimiso Sokusabela
Glycerol + ATP→ Glycerol -3-phosphate + ADP
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe ukuya kokuphuzi kancane, i-lyophilized |
| Umsebenzi | ≥15U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg powder/ml) | Kucace |
| IsiCatalase | ≤0.001% |
| I-glucose oxidase | ≤0.01% |
| Uricase | ≤0.01% |
| I-ATPase | ≤0.005% |
| I-Hexokinase | ≤0.01% |
Ezokuthutha nokugcina
Ezokuthutha:Kuthunyelwa ngaphansi kuka-15°C
Isitoreji :Gcina ku -20°C(Isikhathi eside), 2-8°C(Isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo:Izinyanga ezingu-18
