I-Cholesterol oxidase(COD/CHOD)
Incazelo
I-Cholesterol oxidase (CHOD) ivuselela isinyathelo sokuqala se-cholesterol catabolism.Amanye amagciwane angewona ama-pathogenic, njenge-Streptomyces ayakwazi ukusebenzisa i-cholesterol njengomthombo wekhabhoni.Amagciwane e-Pathogenic, njenge-Rhodococcus equi, adinga i-CHOD ukuthi itheleleke ku-macrophage yomninikhaya.I-CHOD isebenza kabili.I-cholesterol iqala i-oxidized ibe yi-cholest-5-en-3-one esinyathelweni esidinga i-FAD.I-cholest-5-en-3-one ihlukaniswe yaba yi-cholest-4-en3-one.Ukusabela kwe-isomerization kungase kubuyiseleke emuva kancane.Umsebenzi we-CHOD uncike ezintweni ezibonakalayo ze-membrane lapho i-substrate iboshelwe khona.
I-CHOD isetshenziselwa ukunquma i-serum cholesterol.Yi-enzyme yesibili esetshenziswa kakhulu ekuxilongeni ngemuva kwe-glucose oxidase.I-CHOD iphinde ithole ukusetshenziswa ku-microanalysis ye-steroids kumasampula okudla nasekuhlukaniseni i-3-ketosteroids ku-3b-hydroxysteroids.Izitshalo ze-Transgenic eziveza i-cholesterol oxidase ziyaphenywa ekulweni ne-cotton boll weevil.I-Cholesterol oxidase nayo isetshenziswe njengophenyo lwamangqamuzana ukuze kucaciswe ukwakheka kwe-membrane yeselula.
Isakhiwo Samakhemikhali
Isimiso Sokusabela
I-Cholesterol + O2 →△4-Cholesten-3-one + H2O2
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | I-amorphous powder ephuzi, i-lyophilized |
| Umsebenzi | ≥8U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg powder/ml) | Kucace |
| IsiCatalase | ≤0.001% |
| I-glucose oxidase | ≤0.01% |
| I-Cholesterol esterase | ≤0.01% |
| I-ATPase | ≤0.005% |
Ezokuthutha nokugcina
Ezokuthutha:Kuthunyelwe ngaphansi kuka-15°C
Isitoreji :Gcina ku-25~-15°C(Isikhathi eside), 2-8°C(Isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo:1 unyaka
