I-Peroxidase (umthombo we-Horseradish) Igama elifanayo: I-hydrogen peroxide oxidoreductase;HRP

I-Horseradish peroxidase (HRP) ihlukaniswe nezimpande ze-horseradish (Amoracia rusticana) futhi ingeyeqembu le-ferroprotoporphyrin lama-peroxidase.I-HRP ihlangana kalula ne-hydrogen peroxide (H2O2).Umphumela we-[HRP-H2O2] inkimbinkimbi ungafaka oxidize abanikela nge-hydrogen abahlukahlukene:
Umnikezeli + H2O2 → Umnikezeli Owenziwe Nge-Oxidized + 2 H2O
I-HRP izokhipha ama-substrates ahlukahlukene (bona Ithebula 1):
• I-Chromogenic
• I-Chemiluminescent (njenge-luminol noma i-isoluminol)
• I-Fluorogenic (njenge-tyramine, i-homovanillic acid, noma i-4-hydroxyphenyl acetic acid)
I-HRP iyiketango elilodwa le-polypeptide eliqukethe amabhuloho amane e-disulfide.I-HRP iyi-glycoprotein equkethe ama-carbohydrate angu-18%.Ukwakheka kwe-carbohydrate kuhlanganisa i-galactose, i-arabinose, i-xylose, i-fucose, i-mannose, i-mannosamine, ne-galactosamine, kuye ngokuthi i-isozyme ethile.

I-HRP ilebula esetshenziswa kabanzi yama-immunoglobulins ezinhlelweni eziningi ezihlukene ze-immunochemistry, okuhlanganisa i-immunoblotting, i-immunohistochemistry, ne-ELISA.I-HRP ingahlanganiswa nezivikelamzimba ngezindlela eziningana ezihlukene, okuhlanganisa i-glutaraldehyde, i-oxidation yenkathi ethile, ngamabhondi e-disulfide, kanye nangezixhumanisi eziqondiswe ku-amino ne-thiol.I-HRP ilebula efunwa kakhulu yamasosha omzimba, njengoba iyilebula elincane futhi elizinzile kwamalebula e-enzyme amathathu aziwa kakhulu (i-peroxidase, i-β-galactosidase, i-alkaline phosphatase) futhi i-glycosylation yayo iholela ekubopheni okuphansi okungaqondile.Ukubuyekezwa kwe-glutaraldehyde kanye nezindlela zokuhlanganisa zenkathi kushicilelwe.

I-Peroxidase iphinde isetshenziselwe ukunqunywa kwe-glucose4 nama-peroxides esixazululweni.Okushicilelwe okuningana, imiqondo engu-6-24, 25-29 kanye nama-dissertations 30-46 kubalule ukusetshenziswa kwe-P8375 kuzivumelwano zabo zocwaningo.