IGlucose Dehydrogenase (GDH)
Incazelo
I-Glutamate Dehydrogenase (GDH) iyi-enzyme ye-mitochondrial eyenza i-oxidative deamination ye-glutamate ibe yi-a-ketoglutarate futhi isebenza njengesixhumanisi esiyinhloko phakathi kwe-anabolic kanye nezindlela ze-catabolic.Ezilwaneni ezincelisayo, i-GDH ingaphansi kokulawulwa kwe-allosteric futhi inomsebenzi ophezulu esibindini, ezinso, ebuchosheni, nakumanyikwe.Umsebenzi we-GDH ku-serum ungasetshenziselwa ukuhlukanisa phakathi kwezifo zesibindi ngenxa yokuvuvukala kwesibindi, okungabonisi umsebenzi we-GDH we-serum ephakeme, kanye nezifo eziholela ku-hepatocyte necrosis, okuholela ekukhuphukeni kwe-serum GDH.
Umsebenzi we-GDH unqunywa ukuhlolwa kwe-enzyme ehambisanayo lapho i-glutamate isetshenziswa i-GDH ekhiqiza i-NADH, esabela ngophenyo olukhiqiza umkhiqizo we-colorimetric (450 nm) ngokulingana nomsebenzi okhona we-GDH.Iyunithi eyodwa ye-GDH inani le-enzyme ezokhiqiza u-1.0 mmole we-NADH ngomzuzu ku-pH 7.6 ku-37 °C.
Isakhiwo Samakhemikhali
Indlela yokusabela
I-D-Glucose + eyamukelayo → i-D-Glucono-1,5-lactone + isamukeli esincishisiwe
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Impushana emhlophe e-amorphous, i-lyophilized |
| Umsebenzi | ≥160U/mg |
| Ubumsulwa(SDS-PAGE) | ≥90% |
| Ukunyibilika (10 mg powder/mL) | Kucace |
| Ukungcolisa ama-enzyme | |
| IGlucose dehydrogenase (NAD) | ≤0.02% |
| I-Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Ezokuthutha nokugcina
Ezokuthutha: Amaphakethe eqhwa
Isitoreji :Gcina ku-25~-15°C(Isikhathi eside), 2-8°C (isikhathi esifushane)
Ukuhlola kabusha okunconyiweImpilo: Izinyanga ezingu-18
