I-DNase I (Rnase Free) (2u/ul)
Inombolo yekati: HC4007B
I-DNase I iyi-endonuclease ekwazi ukugaya i-DNA enemicu eyodwa noma emibili.Ingakwazi ukwenza i-hydrolyze amabhondi e-phosphodiester ukukhiqiza i-mono-ne-oligodeoxynucleotides equkethe iqembu le-5'-phosphate kanye neqembu le-3'-OH.Ububanzi obulungile be-pH be-DNase I bungu-7-8.Umsebenzi we-DNase I uncike ku-Ca2+futhi ingenziwa isebenze ngama-ion ensimbi e-divalent afana ne-CO2, Mn2+, Zn2+, njll. Phambi kukaMg2+, DNase Ngingakwazi ukuhlukanisa ngokungahleliwe noma iyiphi indawo ye-DNA enemicu ekabili;Ngenkathi ekhona uMn2+, I-DNase Ngingakwazi ukuhlukanisa i-DNA ephindwe kabili endaweni efanayo, yenze iziphetho ezibuthuntu noma iziphetho ezinamathelayo ezinama-nucleotide angu-1-2 aphumele ngaphandle.Ingasetshenziselwa ukucubungula amasampula ahlukahlukene e-RNA.
Izingxenye
| Igama | 1KU | 5KU |
| I-Recombinant DNaseI (i-RNase-free) | 500 μL | 5 ×500 μL |
| I-DNase I Rection Buffer (10×) | 1 mL | 5 × 1mL |
Izimo zokugcina
Lo mkhiqizo kufanele ugcinwe ku -25~-15 ℃ iminyaka engu-2.Sicela ugweme ukuncibilika kweqhwa okuphindaphindiwe.
Iziyalezo
Kusetshenziswa ekukhishweni kwe-DNA kumasampuli e-RNA ukuze kusetshenziswe ireferensi kuphela.
1. Sicela usebenzise amashubhu e-centrifuge angenayo i-RNase namathiphu e-pipette ukuze ulungiselele isistimu yokusabela elandelayo:
| Izingxenye | Ivolumu (μL) |
| I-DNase I Rection Buffer (10×) | 1 |
| I-Recombinant DNasel (i-RNase-free) | 1 |
| I-RNA | X |
| I-ddH yamahhala ye-RNase2O | Kufika ku-10 |
2. Izimo zokusabela zimi kanje: 37℃, ngemva kwemizuzu engu-15-30, engeza ukugxiliswa kokugcina kwesisombululo esingu-2.5 mM EDTA bese uxuba kahle, bese kuba ngu-65℃ imizuzu engu-10.Isifanekiso esicutshunguliwe singasetshenziselwa ukuhlolwa okulandelayo kwe-RT-PCR noma i-RT-qPCR, njll.
Amanothi
1. I-DNase l iyazwela ekujimeni komzimba;Lapho uxuba, hlehlisa ngobumnene ishubhu lokuhlola futhiqhaqhazela kahle, unganyakazi ngamandla.
2. I-enzyme kufanele igcinwe ebhokisini leqhwa noma endaweni yokugeza yeqhwa lapho isetshenziswa, futhi kufanele igcinwe ku -20℃ ngokushesha ngemva kokusetshenziswa.
3. Lo mkhiqizo owokusetshenziswa ucwaningo kuphela.
4. Sicela usebenzise amajazi elebhu namagilavu alahlwayo, ukuze uphephe.
