I-Ribonuclease III (RNase III)
Incazelo
Lo mkhiqizo uyi-ribonuclease III (RNase III) evezwe kabusha ngu-E. coli.Le exonuclease ethize ihlukanisa i-RNA (dsRNA) enemicu ephindwe kabili bese ikhiqiza izingcezu ze-dsRNA ezingu-12-35bp ezinama-5'-PO4 kanye no-3'-OH, 3' ama-overhangs.
Isakhiwo Samakhemikhali
Incazelo Yeyunithi
Incazelo yeyunithi yomsebenzi: Iyunithi eyodwa ibhekisela enanini le-enzyme edingekayo ukuze kwehliswe i-1 μg ye
I-dsRNA iye ku-siRNA kusistimu yokusabela engu-50 μL ku-37°C imizuzu engu-20.
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Umsebenzi we-Exonuclease | Ukukhishwa＜ 0.1% wesamba se-radioactivity |
| Umsebenzi Ongaqondile We-Dnase | Ayibonakali |
| I-Protein Purity Assay(SDS-PAGE) | ≥ 95% |
| Umsebenzi we-RNase (Ukugaya Okunwetshiwe) | ＞90% we-substrate RNA ihlala injalo |
Ezokuthutha nokugcina
Ezokuthutha:Iqhwa elomile
Isitoreji:Gcina ku -25~-15°C
Impilo yokuhlola kabusha enconyiwe:2 iminyaka
imikhiqizo ehlobene
Bhala umyalezo wakho lapha futhi usithumelele wona
