I-RT-LAMP Colormetric (ibhola le-Lyophilized)

Lo mkhiqizo uqukethe isivimbeli sokusabela, i-RT-Enzymes Mix (i-Bst DNA polymerase kanye ne-reverse transcriptase engashi), izivikeli ezi-lyophilized kanye nezingxenye zikadayi ze-chromogenic.Umkhiqizo uwuhlobo lwebhola le-lyophilized, usebenzisa kuphela ama-primers nezifanekiso.Le kit inikeza ukutholwa okubonakalayo okusheshayo, okucacile kokukhuliswa, ikuphi ukusabela okungalungile okuboniswa ngokubomvu nokusabela okuhle kuboniswa ukushintsha kokuphuzi.

Isakhi

I-RT-LAMP Colormetric Master Mix (ubuhlalu be-Lyophilized)

Izinhlelo zokusebenza

Nge-DNA noma i-RNA isothermal amplification.

Izimo Zokugcina

Kuthuthwa futhi kugcinwe ku-2 ~ 8℃.Umkhiqizo usebenza izinyanga eziyi-12.

Iphrothokholi

1.Khipha inombolo ehambisanayo yobuhlalu be-Lyophilized powder ngokwenani lokuhlolwa.

2.Lungiselela imiksi yokusabela

Amanothi:

1. 10×I-Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

2. Izifanekiso ze-Nucleic acid zinconywa ukuthi zihlakazwe kusetshenziswa amanzi e-DEPC.

3.Fukamela ku-65°C imizuzu engu-30-45, enganwetshwa ngokufanele ngokuya ngokushintsha kombala Isikhathi sokusabela.

4.Ngokweso lenyama, ophuzi ube positive kuthi obomvu ube negative.

Amanothi

1.Izinga lokushisa lokusabela lingenziwa kahle phakathi kuka-62 ℃ no-68 ℃ ngokwesimo sokuqala.

2.Ama-reagents ahlanganisiwe akufanele avezwe emoyeni isikhathi eside.

3.Ukusabela kokuguquka kombala okubomvu nokuphuzi kuncike ekushintsheni kwe-pH yesistimu yokusabela, sicela ungasebenzisi isisombululo sokugcina i-Tris nucleic acid esiqukethe, esinconyelwe ukusebenzisa i-ddH.₂O egcinwe i-nucleic acid.

4.Ukuhlolwa kuzokwenziwa ngendlela emisiwe, okuhlanganisa ukulungiswa kwesistimu yokusabela, ukwelashwa kwesampula kanye nokwengezwa kwesampula.

5.Kunconywa ukuthi kulungiswe isistimu yokusabela kuthebula elihlanzeke kakhulu futhi kwengezwe izifanekiso ku-fume hood yamanye amagumbi ukuze kugwenywe amanga.