I-M-MLV Reverse Transcriptase (Glycerol free) HC2005A Isithombe Esifakiwe

I-M-MLV Reverse Transcriptase (iGlycerol free)

Inombolo yekati:HC2005A

Iphakheji: 10000U/40000U

I-lyophilizable Reverse Transcriptase.Ingasetshenziswa kubuchwepheshe be-lyophilization obuya phansi ngenkathi igcinwa ukusebenza okuhle kokuhlehla kokulotshwa nokuzinza.

Thumela Uphenyo

Incazelo Yomkhiqizo

Imininingwane yomkhiqizo

I-lyophilizable Reverse Transcriptase.Ingasetshenziswa kubuchwepheshe be-lyophilization obuya phansi ngenkathi igcinwa ukusebenza okuhle kokuhlehla kokulotshwa nokuzinza.Lo mkhiqizo awunazo izinto ezisizayo, sicela wengeze eyakho njengoba kudingeka.

Okwedlule: I-M-MLV Neoscript Reverse Transcriptase HC2004A

Olandelayo: I-dA/T/U/GP (100mM)I-UltraPure HC2201A

Izingxenye

Isakhi

HC2005A-01

(10,000U)

HC2005A-02

(40,000U)

I-Reverse Transcriptase (I-Glycerol Free) (200U/μL)

50 μL

200 μL

5 × Isilondolozi

200 μL

800 μL

Isicelo:

Isebenza ekuphenduleni kwe-RT-qPCR okuyisinyathelo esisodwa.

Isimo Sesitoreji

Gcina ku -30 ~ -15°C futhi ukuthutha ku-≤0°C.

Incazelo Yeyunithi

Iyunithi eyodwa (U) ichazwa njengenani le-enzyme elihlanganisa i-1 nmol ye-dTTP kwinto engancibiliki i-asidi ku-10mins ku-37°C, ne-Poly(rA)·Oligo (dT) njengesifanekiso/i-primer.

Amanothi

Okokusetshenziswa kocwaningo kuphela.Ayisetshenziselwa izinqubo zokuxilonga.

1.Sicela ugcine indawo yokuhlola ihlanzekile;Gqoka amagilavu namamaski alahlwayo;Sebenzisa izinto ezidlekayo ezingenawo i-RNase njengamashubhu e-centrifuge namathiphu e-pipette.

2.Gcina i-RNA eqhweni ukuze ugweme ukucekelwa phansi.

3.Izifanekiso ze-RNA zekhwalithi ephezulu ziyanconywa ukuze kuzuzwe ukulotshwa okusebenzayo okuhlehliswayo okuphezulu.