I-Hotstart Taq DNA Polymerase (5u/ul)
I-Taq DNA Polymerase iyisiqalo esishisayo se-DNA polymerase evinjwa kabili ngamasosha omzimba aphindwe kabili.Lo mkhiqizo awuvimbi kuphela umsebenzi we-5′→3′ polymerase we-Taq DNA polymerase, kodwa futhi uvimba umsebenzi we-5′→3′exonuclease.Ukushisa amasekhondi angu-30 ezingeni lokushisa langaphambi kwe-denaturation kungenza i-antibody ngokuphelele futhi kukhulule umsebenzi we-DNA polymerase nomsebenzi we-exonuclease.Isici sokuvinjwa kabili asikwazi nje kuphela ukuvimbela ngempumelelo ukukhulisa okungaqondile okubangelwa ukungafani noma i-primer dimer, kodwa futhi zivimbele ngempumelelo ukwehla kwesiginali ye-fluorescence okubangelwa ukuwohloka kwe-probe, ukuze yenze i-reagent yokuthola i-in vitro izinze kakhudlwana ngesikhathi sokuthutha noma ukusetshenziswa ekamelweni. izinga lokushisa.
Izingxenye
| Isakhi | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| I-Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 ml | 5 ml |
Isimo Sesitoreji
Umkhiqizo uthunyelwa ngeqhwa elomile futhi ungagcinwa ku -25°C~-15°C iminyaka engu-2.
Imininingwane
| I-Polymerase | I-Taq DNA Polymerase |
| Ubumsulwa | ≥ 95% (SDS-PAGE) |
| Isiqalo Esishisayo | I-Hot Start eyakhelwe ngaphakathi |
| Isivinini Sokusabela | Okujwayelekile |
| Umsebenzi we-Exonuclease | 5′→3′ |
Iziyalezo
Ukusethwa kokusabela
| Izingxenye | Ivolumu (μL) | Ukugxila Kokugcina |
| 2× Isivimbelia | 25 | 1× |
| I-Primer/Probe mixb | × | 0.1 μmol/L-0.5 μmol/L |
| I-Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Isifanekiso se-DNAc | × | 0.1-100 ng |
| ddH2O | Kufika ku-50 | - |
Amanothi:
1) Ngokohlelo lokusebenza oluthile lokuhlola, kuyadingeka ukuze kulungiswe isigcinalwazi esihambelanayo.
I-2) Inani le-DNA kanye nokuhlushwa kwama-probes noma ama-primers kunconywa ukugxila.Ukugxilisa ingqondo okuphelele kungalungiswa ngokuya ngezimo ezithile zokuhlola.
Iphrothokholi yokuhamba ngebhayisikili eshisayo
| Isinyathelo | Izinga lokushisa(°C) | Isikhathi | Imijikelezo |
| I-pre-denaturation | 95 ℃ | 5 imiz | 1 |
| I-Denaturation | 95 ℃ | 15 isekhondi | 45 |
| I-Annealing / Isandiso | 60 ℃a | 30 isekhondib |
Amanothi:
I-1) Izinga lokushisa lokusabela lilungiswa ngokuya ngenani le-Tm lama-primer aklanyelwe.
2) Amathuluzi ahlukene e-qPCR adinga isikhathi sokutholwa kwesiginali ye-fluorescence ehlukile, sicela usethe ngokuya ngomkhawulo wesikhathi omfushane kakhulu.
Amanothi
Sicela ugqoke i-PPE edingekayo, ijazi lelebhu kanye namagilavu, ukuze uqinisekise impilo nokuphepha kwakho!
