Isinyathelo esisodwa RT-qPCR SYBR Green Kit

I-Universal Blue qPCR Master Mix (i-Dye Based) iyisixazululo sangaphambilini sokukhuliswa komthamo we-PCR wesikhathi sangempela esingu-2x esibonakala ngokuzwela okuphezulu nokucaciswa kwawo, siluhlaza okwesibhakabhaka ngombala, futhi sinomphumela wokulandela umkhondo wokwengezwa kwesampula.Ingxenye eyinhloko ye-Taq DNA polymerase isebenzisa isiqalo esishisayo se-antibody ukuze ivimbele ngempumelelo ukukhuliswa okungaqondile ngenxa yokufakwa kwe-primer ngesikhathi sokulungiselela isampula.Ngasikhathi sinye, ifomula yengeza izici ezikhuthazayo zokuthuthukisa ukusebenza kahle kokukhulisa ukusabela kwe-PCR nokulinganisa ukukhuliswa kofuzo ngokuqukethwe okuhlukile kwe-GC (30 ~ 70%), ukuze i-PCR yobuningi ikwazi ukuthola ubudlelwano obuhle bomugqa ngobuningi obubanzi. isifunda.Lo mkhiqizo uqukethe i-ROX Passive Reference Dye ekhethekile, esebenza kuwo wonke amathuluzi e-qPCR.Akudingekile ukulungisa ukugcwala kwe-ROX kumathuluzi ahlukene.Kudingeka kuphela ukwengeza ama-primers namathempulethi ukuze ulungiselele uhlelo lokusabela lokukhulisa.