I-RT-LAMP Fluorescent (ibhola le-Lyophilized)
I-RT-LAMP Fluorescent Master Mix (Lyophilized Powder) iqukethe isigcinalwazi sokusabela, i-Bst DNA polymerase kanye ne-reverse transcriptase engazweli ukushisa, isivikeli se-lyophilized, kanye nodayi we-fluorescent.Umkhiqizo usesimweni sobuhlalu be-lyophilized, futhi ama-primers nezifanekiso kuphela ezidinga ukungezwa ukuze zisetshenziswe.
Ingxenye ye-Reagent
I-RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Ukusetshenziswa
Nge-DNA noma i-RNA isothermal amplification
Izimo Zokugcina
Ezokuthutha kanye nesitolo ku-2~8°C.
Impilo yeshelufu: izinyanga eziyi-12.
Iphrothokholi
1. Khipha inombolo ehambisanayo yobuhlalu obu-lyophilized ngokwenani lokuhlolwa.
2. Ukulungiselela uhlelo lokusabela
| Isakhi | Umthamo |
| I-RT-LAMP Fluorescent Master Mix (I-Lyophilized Powder) | 2 ubuhlalu |
| Udayi we-fluorescent | x uml |
| 10 × I-Primer Mix a | 5 μl |
| Izifanekiso DNA/RNA b | n uml |
| Isamba | 50 μL |
Amanothi:
1) a.10×I-Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Iluphu F/B;
2) b.Isifanekiso se-nucleic acid sinconywa ukuthi sihlakazwe emanzini e-DEPC.
I-Hyasen Biotechnology Co., Ltd
3) Uhlelo lokusabela
| Izinga lokushisa | Isikhathi | Imijikelezo |
| 65℃ | 60s a | 30 |
Amanothi:
1. Qoqa uphawu lwe-fluorescent
Amanothi
1. Izinga lokushisa lokusabela lingenziwa kahle phakathi kuka-62°C no-68°C ngokusho kwe-primerizimo;
2. Ukuhlolwa kufanele kumise ukusebenza, okuhlanganisa ukulungiswa kwesistimu yokusabela,ukucubungula isampula kanye nomthamo;
3. Ukuze ugweme ukungcola, kunconywa ukuthi ulungise uhlelo lokusabela etafuleni elihlanzekile kakhulufuthi wengeze izifanekiso ku-fume hood kwamanye amagumbi ukuze ugweme ukuphazamiseka okungelona iqiniso.
