Ikhithi ye-Ultra Nuclease Assay(ELISA) Isithombe Esifakiwe

I-Ultra Nuclease Assay Kit(ELISA)

Inombolo yeCas:9025-65-4

EC.Inombolo yomthetho: 3.1.30.2

Iphakheji: 96 Test

Thumela Uphenyo

Imininingwane Yomkhiqizo

Incazelo

Le kit ye-Ultra Nuclease ELISA iyisihlabathi i-ELISA okufanele yenziwe ngefomethi ye-microplate.Amasampula okungenzeka aqukathe i-endonuclease afakwe emithonjeni yamapuleti e-microtiter ambozwe ngaphambili nge-anti-endonuclease capture antibody ehlanzekile.Ngemuva kokufakwa kanye nesinyathelo sokugeza lapho kukhishwa khona izingxenye ezingaboshiwe, i-enzyme-conjugated, i-anti-endonuclease detector antibody iyengezwa.Ukusabela kwe-immunological kubangela ukwakheka kwesamishi eliyinkimbinkimbi yesigaba esiqinile se-antibody-endonuclease-enzyme ebizwa ngokuthi i-antibody.Ngemuva kwesinyathelo sokugcina sokugeza, isisombululo se-substrate sengezwa emithonjeni futhi siphendule, okuholela ekuthuthukisweni kombala.Ukuminyana kokubona kukalwa nge-photometric futhi kuhambisana ne-
i-analyte concentration ekhona emithonjeni.Ukugxiliswa kwe-endonuclease kumasampuli angaziwa kungabalwa ngokusekelwe kwijika elijwayelekile elihambisanayo.

Isakhiwo Samakhemikhali

Incazelo Yeyunithi

Inani le-enzyme elisetshenziselwa ukushintsha inani lokumunca lika-△A260 ngo-1.0 phakathi kwemizuzu engama-30
ku-37 °C, i-pH 8.0, okulingana ne-DNA yesidoda se-salmon engu-37μg ngokusikwa ku-oligonucleotides, yachazwa njengeyunithi esebenzayo.

Ukusetshenziswa kanye Nesilinganiso

• Khipha i-nucleic acid yangaphandle emikhiqizweni yokugoma, nciphisa ingcuphe yensalela yobuthi be-nucleic acid futhi uthuthukise ukuphepha komkhiqizo.
• Yehlisa i-viscosity yoketshezi lwe-feed olubangelwa i-nucleic acid, nciphisa isikhathi sokucubungula futhi andise isivuno samaprotheni.
• Khipha i-nucleic acid egoqiwe izinhlayiyana (igciwane, umzimba wokufakwa, njll.), esiza ekukhululweni nasekuhlanzweni kwezinhlayiyana.
• Ukwelashwa kwe-nuclease kungathuthukisa ukuxazululwa nokutholwa kwesampula yekholomu ye-chromatography, i-electrophoresis kanye nokuhlaziywa kwe-blotting.
• Ekwelashweni kofuzo, i-nucleic acid iyasuswa ukuze kutholwe amagciwane ahlotshaniswa ne-adeno.