I-Alkaline Phosphatase(ALP)

I-Alkaline Phosphatase isuselwa ohlotsheni oluhlanganisiwe lwe-E. coli oluthwala isakhi sofuzo se-TAB5.I-enzyme ivuselela i-dephosphorylation ye-5' kanye ne-3' ekupheleni kwe-DNA kanye ne-RNA phosphomonoesters.Futhi, i-hydrolyses i-ribose, kanye ne-deoxyribonucleoside triphosphates (NTPs kanye nama-dNTPs).I-TAB5 i-Alkaline Phosphatase isebenza ekuphumeni okungu-5, oku-5' kokuhlehla kanye neziphetho ezibuthuntu.I-Phosphatase ingasetshenziswa ezinhlelweni eziningi zebhayoloji yamangqamuzana, njengokuhlanganisa i-cloning noma ukulebula kokuphela kwe-probe ukuze kukhishwe iziphetho ezine-phosphorylated ze-DNA noma i-RNA.Ekuhlolweni kwe-cloning, i-dephosphorylation ivimbela i-DNA ye-plasmid eqondile ekuzibopheni.Ingase futhi yehlise izinga le-dNTP engahlanganisiwe ekuphenduleni kwe-PCR ukuze ilungiselele isifanekiso sokulandelana kwe-DNA.I-enzyme yenziwe ingasebenzi ngokuphelele futhi ngokungenakuhlehliswa ngokushisisa ku-70°C imizuzu emi-5, ngaleyo ndlela yenze ukususwa kwe-phosphatase ngaphambi kokuxhunywa noma ukuqeda ukulebula kungenasidingo.