I-Ultra Nuclease
Incazelo
I-UltraNuclease i-endonuclaese eyenziwe ngofuzo etholakala ku-Serratia marcescens, ekwazi ukuthunaza i-DNA noma i-RNA, okuphindwe kabili noma okukodwa, okuqondile noma okuyisiyingi ngaphansi kwezimo eziningi ezahlukene, yehlisa ngokuphelele ama-nucleic acid abe yi-5'-monophosphate oligonucleotides ene-3-5. ubude besisekelo.
Ngemva kokuguqulwa kobunjiniyela bezakhi zofuzo, umkhiqizo wavutshelwa, wavezwa, futhi wahlanzwa ku-Escherichia coli (E. coli), okunciphisa i-viscosity ye-cell supernatant kanye ne-cell lysate ocwaningweni lwesayensi, kodwa futhi kuthuthukise ukusebenza kahle kokuhlanzwa kanye nocwaningo olusebenzayo lwamaprotheni.Ingasetshenziswa futhi ekwelashweni kwezakhi zofuzo, ukuhlanzwa kwegciwane, ukukhiqizwa kokugoma, amaprotheni kanye nemboni yezemithi ye-polysaccharide njenge-reagent yokususa izinsalela ze-nucleic acid.
Isakhiwo Samakhemikhali
Incazelo Yeyunithi
Inani le-enzyme elisetshenziselwa ukuguqula inani lokumunca lika-△A260 ngo-1.0 phakathi kwemizuzu engama-30 ku-37 °C, i-pH 8.0, elilingana ne-37μg yesidoda se-salmon ye-DNA ngokusikwa kube yi-oligonucleotides, lichazwe njengeyunithi esebenzayo.
Ukusetshenziswa kanye Nesilinganiso
• Khipha i-nucleic acid yangaphandle emikhiqizweni yokugoma, nciphisa ingcuphe yensalela yobuthi be-nucleic acid futhi uthuthukise ukuphepha komkhiqizo.
• Yehlisa i-viscosity yoketshezi lwe-feed olubangelwa i-nucleic acid, nciphisa isikhathi sokucubungula futhi andise isivuno samaprotheni.
• Khipha i-nucleic acid egoqiwe izinhlayiyana (igciwane, umzimba wokufakwa, njll.), esiza ekukhululweni nasekuhlanzweni kwezinhlayiyana.
• Ukwelashwa kwe-nuclease kungathuthukisa ukuxazululwa nokutholwa kwesampula yekholomu ye-chromatography, i-electrophoresis kanye nokuhlaziywa kwe-blotting.
• Ekwelashweni kofuzo, i-nucleic acid iyasuswa ukuze kutholwe amagciwane ahlotshaniswa ne-adeno.
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Incazelo | Icacile futhi Ayinambala |
| Umsebenzi | ≥ 250 U/ul |
| Umsebenzi othize | ≥1.1*106U/mg |
| Ukuhlanzeka (SDS-PAGE) | ≥ 99.0% |
| AmaProtease | Akukho okutholiwe |
| I-Bioburden | ＜10 cfu/100,000U |
| I-Endotoxines(LAL-test) | ＜ 0.25EU/1,000U |
Ezokuthutha nokugcina
Ezokuthutha:Kuthunyelwe ngaphansi kuka-0 °C
Isitoreji:Gcina ku -25~-15°C
Impilo yokuhlola kabusha enconyiwe:Iminyaka emi-2 (gwema ukuncibilika okokuqandisa)
