Isinyathelo esisodwa se-RT-qPCR probe kit
Incazelo
I-One Step qRT-PCR Probe Kit yakhelwe ngokukhethekile i-qPCR esebenzisa ngokuqondile i-RNA (isb. i-virus RNA) njengesifanekiso.Kusetshenziswa iziqalo eziqondile zofuzo (GSP), ukuloba okuhlanekezelwe kanye ne-qPCR kungaqedwa ngeshubhu eyodwa, kwehlise kakhulu izinqubo zamapayipi kanye nobungozi bokungcoliswa.Ingenziwa ingasebenzi ku-55℃ ngaphandle kokuthikameze ukusebenza kahle nokuzwela kwe-qRT-PCR.Isinyathelo esisodwa se-qRT-PCR Probe Kit sinikezwa nge-Master Mix.I-5 × One Step Mix iqukethe ibhafa ethuthukisiwe kanye ne-dNTP/dUTP Mix, futhi ilungele amasistimu okuthola okukhethekile asekelwe kuma-fluorescence anelebuli probe (isb. TaqMan).
Izimiso Eziyisisekelo ze-RT-qPCR
Ukucaciswa
| Izinto Zokuhlola | Ukucaciswa | Umphumela |
| (SDS IKHASI) Ukuhlanzeka Kwesitoko Se-Enzyme(SDS IKHASI) | ≥95% | Dlula |
| Umsebenzi we-Endonuclease | Ayitholakali | Dlula |
| Umsebenzi we-Exodulease | Ayitholakali | Dlula |
| Umsebenzi we-Rnase | Ayitholakali | Dlula |
| I-Residual E.coli DNA | ＜1 amakhophi/60 | Dlula |
| I-Functional Assay-System | 90%≤110% | Dlula |
Izingxenye
| Izingxenye | 100rxns | 1,000rns | 5,000 rxns |
| I-ddH2O yamahhala ye-RNase | 2*1ml | 20ml | 100ml |
| 5*isinyathelo esisodwa sokuxuba | 600μl | 6*1ml | 30ml |
| Isinyathelo esisodwa sokuxuba i-enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX inkomba Udayi 1 | 60μl | 600μl | 3*1ml |
| 50* ROX inkomba Udayi 2 | 60μl | 600μl | 3*1ml |
a.I-One-step Buffer ihlanganisa i-dNTP Mix ne-Mg2+.
b.I-Enzyme Mix ikakhulukazi iqukethe okuphambene
transcriptase, Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kanye ne-RNase inhibitor.
c.Isetshenziselwa ukulungisa iphutha lezinhlayiya ze-fluorescene phakathi kwemithombo ehlukene.
d.I-ROX: Udinga ukukhetha ukulinganisa ngokuya ngemodeli yethuluzi lokuhlola.
Izinhlelo zokusebenza
Ukutholwa kwe-QPCR
Ukuthumela kanye Nesitoreji
Ukuthumela:Amaphakethe eqhwa
Imibandela Yesitoreji:Gcina ku -20 ℃.
I-Shief Life:Izinyanga ezingu-18
