I-ELEISA KIT ye-Trypsin
Incazelo
I-recombinant trypsin ivamise ukusetshenziswa ekukhiqizeni i-biopharmaceutical-ngesikhathi sokulungiswa kwamaseli noma ukuguqula nokwenza kusebenze imikhiqizo.I-Trypsin idala izingozi zokuphepha ngakho-ke kufanele isuswe ngaphambi kokukhishwa komkhiqizo kokugcina.Le Kit Sandwich ingeyesilinganiso sobuningi se-Residual Trypsin ku-cell culture supernatant kanye nezinye izinqubo zokwenziwa kwe-biopharmaceutical lapho kusetshenziswa i-Trypsin.
Le kit iyi-Enzyme-Linked Immunosorbent Assay (ELISA).Ipuleti limbozwe ngaphambili nge-Porcine trypsin antibody.I-Trypsin ekhona kusampula iyengezwa futhi ibophezela kumasosha omzimba ambozwe emithonjeni.Bese kwengezwa i-biotinylated i-Porcine trypsin antibody futhi ibophezela ku-trypsin kusampula.Ngemva kokuwashwa, i-HRP-Streptavidin iyengezwa futhi ibophezela ku-Biotinylated trypsin antibody.Ngemuva kokufukamela, i-HRP-Streptavidin iyagezwa.Bese kwengezwa isixazululo se-TMB substrate futhi senziwa i-HRP ukuze kukhiqizwe umkhiqizo onemibala eluhlaza okwesibhakabhaka oshintshe waba ophuzi ngemva kokwengeza isixazululo sokumisa i-acidic.Ubuningi bombala ophuzi bulingana nenani eliqondiwe le-trypsin
isampula ethathwe epuletini.I-absorbence ilinganiswa ku-450 nm.
Isakhiwo Samakhemikhali
Ukucaciswa
| Izinto Zokuhlola | Imininingwane |
| Ukubukeka | Ukupakisha okuphelele futhi akukho ukuvuza okuwuketshezi |
| Umkhawulo ophansi wokutholwa | 0.003 ng/mL |
| Umkhawulo ophansi wenani | 0.039 ng/mL |
| Ukunemba | I-Intra assay CV≤10% |
Ezokuthutha nokugcina
Ezokuthutha:I-ambient
Isitoreji:Ingagcinwa ku -25~-15°C empilweni yeshelufu, 2-8°C ukuze uthole okunye ukuhlola kalula
Impilo yokuhlola kabusha enconyiwe:1 unyaka
