Isinyathelo esisodwa se-RT-qPCR probe kit

I-One Step qRT-PCR Probe Kit yakhelwe ngokukhethekile i-qPCR esebenzisa ngokuqondile i-RNA (isb. i-virus RNA) njengesifanekiso.Kusetshenziswa iziqalo eziqondile zofuzo (GSP), ukuloba okuhlanekezelwe kanye ne-qPCR kungaqedwa ngeshubhu eyodwa, kwehlise kakhulu izinqubo zamapayipi kanye nobungozi bokungcoliswa.Ingenziwa ingasebenzi ku-55℃ ngaphandle kokuthikameze ukusebenza kahle nokuzwela kwe-qRT-PCR.Ihlanganisa ukusebenza okuphezulu kwe-HiScript III Reverse Transcriptase kanye ne-Champagne Taq DNA Polymerase eshisa izikhotha, enesistimu yokubhafa ethuthukisiwe, ukuzwela kokutholwa kwe-One Step qRT-PCR Probe Kit kungafinyelela ku-0.1 pg wesamba se-RNA noma amakhophi angaphansi kwayi-10 ezifanekiso ze-RNA. futhi inokuqina okuphezulu kokushisa.Isinyathelo esisodwa se-qRT-PCR Probe Kit sinikezwa nge-Master Mix.I-5 × One Step Mix iqukethe ibhafa ethuthukisiwe kanye ne-dNTP/dUTP Mix, futhi ilungele amasistimu okuthola okukhethekile asekelwe kuma-fluorescence anelebuli probe (isb. TaqMan).